Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction
It is not clear which designs, other than completely randomized ones, are valid for scRNA-seq experiments so that batch effects can be adjusted. Here the authors show that under flexible reference panel and chain-type designs, biological variability can also be separated from batch effects, at least...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-16905-2 |
| _version_ | 1818683771025620992 |
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| author | Fangda Song Ga Ming Angus Chan Yingying Wei |
| author_facet | Fangda Song Ga Ming Angus Chan Yingying Wei |
| author_sort | Fangda Song |
| collection | DOAJ |
| description | It is not clear which designs, other than completely randomized ones, are valid for scRNA-seq experiments so that batch effects can be adjusted. Here the authors show that under flexible reference panel and chain-type designs, biological variability can also be separated from batch effects, at least by BUSseq. |
| first_indexed | 2024-12-17T10:40:02Z |
| format | Article |
| id | doaj.art-889067254b4d4524ba0685f276a62942 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T10:40:02Z |
| publishDate | 2020-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-889067254b4d4524ba0685f276a629422022-12-21T21:52:17ZengNature PortfolioNature Communications2041-17232020-07-0111111510.1038/s41467-020-16905-2Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correctionFangda Song0Ga Ming Angus Chan1Yingying Wei2Department of Statistics, The Chinese University of Hong KongDepartment of Statistics, The Chinese University of Hong KongDepartment of Statistics, The Chinese University of Hong KongIt is not clear which designs, other than completely randomized ones, are valid for scRNA-seq experiments so that batch effects can be adjusted. Here the authors show that under flexible reference panel and chain-type designs, biological variability can also be separated from batch effects, at least by BUSseq.https://doi.org/10.1038/s41467-020-16905-2 |
| spellingShingle | Fangda Song Ga Ming Angus Chan Yingying Wei Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction Nature Communications |
| title | Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction |
| title_full | Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction |
| title_fullStr | Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction |
| title_full_unstemmed | Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction |
| title_short | Flexible experimental designs for valid single-cell RNA-sequencing experiments allowing batch effects correction |
| title_sort | flexible experimental designs for valid single cell rna sequencing experiments allowing batch effects correction |
| url | https://doi.org/10.1038/s41467-020-16905-2 |
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