Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simpl...

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Hlavní autoři: Katharine H. D. Crawford, Rachel Eguia, Adam S. Dingens, Andrea N. Loes, Keara D. Malone, Caitlin R. Wolf, Helen Y. Chu, M. Alejandra Tortorici, David Veesler, Michael Murphy, Deleah Pettie, Neil P. King, Alejandro B. Balazs, Jesse D. Bloom
Médium: Článek
Jazyk:English
Vydáno: MDPI AG 2020-05-01
Edice:Viruses
Témata:
SARS-CoV-2
COVID-19
coronavirus
neutralization assay
lentiviral pseudotype
Spike
On-line přístup:https://www.mdpi.com/1999-4915/12/5/513

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https://www.mdpi.com/1999-4915/12/5/513

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