In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces
The chemical composition of berries and berry pomaces is diverse, containing polyphenolic components that may have both antibacterial and antioxidative properties. In the present study, in vitro antibacterial effect of the extracts of chokeberry, blackcurrant, and rowan berries and berry pomaces aga...
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MDPI AG
2024-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/3/421 |
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| author | Kadrin Meremäe Piret Raudsepp Linda Rusalepp Dea Anton Uko Bleive Mati Roasto |
| author_facet | Kadrin Meremäe Piret Raudsepp Linda Rusalepp Dea Anton Uko Bleive Mati Roasto |
| author_sort | Kadrin Meremäe |
| collection | DOAJ |
| description | The chemical composition of berries and berry pomaces is diverse, containing polyphenolic components that may have both antibacterial and antioxidative properties. In the present study, in vitro antibacterial effect of the extracts of chokeberry, blackcurrant, and rowan berries and berry pomaces against <i>L. monocytogenes</i>, <i>S. aureus</i>, <i>E. coli</i>, and <i>C. jejuni</i> was studied. In addition, the polyphenolic profile and antioxidant activity of these extracts were investigated. The polyphenolic profiles in the aqueous and 30% ethanolic extracts were determined chromatographically by HPLC-MS, and the total polyphenol content was estimated spectrophotometrically by HPLC-DAD-UV. The minimal inhibition concentrations (MICs) of the extracts against tested bacteria were determined by the broth microdilution method. The content of total polyphenols was highest and good antioxidative properties of the extracts were determined for chokeberry and blackcurrant berries and their pomaces. The highest proportions of total quercetin derivatives and anthocyanins were found in the extracts of chokeberry berry/pomace and blackcurrant berry/pomace, respectively. The sensitivity of tested microbes to the extracts of berries and berry pomaces was as follows: <i>S. aureus</i> > <i>L. monocytogenes</i> > <i>E. coli</i> and <i>C. jejuni.</i> In vitro antibacterial activity of tested extracts depended on the extraction solvent, mainly for the ethanolic extracts. Findings suggest that chokeberry and blackcurrant berries and their pomaces can be used as a good source of polyphenols with antioxidative properties, and they also have antibacterial activity against some foodborne pathogenic bacteria. It is important that the valuable compounds are extracted from juice press residues before their disposal. |
| first_indexed | 2024-03-08T03:57:49Z |
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| last_indexed | 2024-03-08T03:57:49Z |
| publishDate | 2024-01-01 |
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| series | Foods |
| spelling | doaj.art-88b037e7066f400096a6185d0d2ff8892024-02-09T15:12:09ZengMDPI AGFoods2304-81582024-01-0113342110.3390/foods13030421In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their PomacesKadrin Meremäe0Piret Raudsepp1Linda Rusalepp2Dea Anton3Uko Bleive4Mati Roasto5Chair of Veterinary Biomedicine and Food Hygiene, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 56/3, 51006 Tartu, EstoniaChair of Veterinary Biomedicine and Food Hygiene, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 56/3, 51006 Tartu, EstoniaChair of Veterinary Biomedicine and Food Hygiene, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 56/3, 51006 Tartu, EstoniaChair of Veterinary Biomedicine and Food Hygiene, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 56/3, 51006 Tartu, EstoniaPolli Horticultural Research Centre, Chair of Horticulture, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Uus 2, 69108 Polli, EstoniaChair of Veterinary Biomedicine and Food Hygiene, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Fr. R. Kreutzwaldi 56/3, 51006 Tartu, EstoniaThe chemical composition of berries and berry pomaces is diverse, containing polyphenolic components that may have both antibacterial and antioxidative properties. In the present study, in vitro antibacterial effect of the extracts of chokeberry, blackcurrant, and rowan berries and berry pomaces against <i>L. monocytogenes</i>, <i>S. aureus</i>, <i>E. coli</i>, and <i>C. jejuni</i> was studied. In addition, the polyphenolic profile and antioxidant activity of these extracts were investigated. The polyphenolic profiles in the aqueous and 30% ethanolic extracts were determined chromatographically by HPLC-MS, and the total polyphenol content was estimated spectrophotometrically by HPLC-DAD-UV. The minimal inhibition concentrations (MICs) of the extracts against tested bacteria were determined by the broth microdilution method. The content of total polyphenols was highest and good antioxidative properties of the extracts were determined for chokeberry and blackcurrant berries and their pomaces. The highest proportions of total quercetin derivatives and anthocyanins were found in the extracts of chokeberry berry/pomace and blackcurrant berry/pomace, respectively. The sensitivity of tested microbes to the extracts of berries and berry pomaces was as follows: <i>S. aureus</i> > <i>L. monocytogenes</i> > <i>E. coli</i> and <i>C. jejuni.</i> In vitro antibacterial activity of tested extracts depended on the extraction solvent, mainly for the ethanolic extracts. Findings suggest that chokeberry and blackcurrant berries and their pomaces can be used as a good source of polyphenols with antioxidative properties, and they also have antibacterial activity against some foodborne pathogenic bacteria. It is important that the valuable compounds are extracted from juice press residues before their disposal.https://www.mdpi.com/2304-8158/13/3/421antibacterial effectMICantioxidative activityphenolic compoundschokeberryblackcurrant |
| spellingShingle | Kadrin Meremäe Piret Raudsepp Linda Rusalepp Dea Anton Uko Bleive Mati Roasto In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces Foods antibacterial effect MIC antioxidative activity phenolic compounds chokeberry blackcurrant |
| title | In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces |
| title_full | In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces |
| title_fullStr | In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces |
| title_full_unstemmed | In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces |
| title_short | In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces |
| title_sort | in vitro antibacterial and antioxidative activity and polyphenolic profile of the extracts of chokeberry blackcurrant and rowan berries and their pomaces |
| topic | antibacterial effect MIC antioxidative activity phenolic compounds chokeberry blackcurrant |
| url | https://www.mdpi.com/2304-8158/13/3/421 |
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