Glycosylation network mapping and site-specific glycan maturation in vivo
Summary: Glycoprotein processing along a complex highly compartmentalized pathway is a hallmark of eukaryotic cells. We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretor...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222016893 |
| _version_ | 1828095508467417088 |
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| author | Marie-Estelle Losfeld Ernesto Scibona Chia-wei Lin Markus Aebi |
| author_facet | Marie-Estelle Losfeld Ernesto Scibona Chia-wei Lin Markus Aebi |
| author_sort | Marie-Estelle Losfeld |
| collection | DOAJ |
| description | Summary: Glycoprotein processing along a complex highly compartmentalized pathway is a hallmark of eukaryotic cells. We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretory pathway that describes a complex set of processing reactions localized in defined intracellular compartments such as the endoplasmic reticulum the Golgi, or the lysosome. The model was able to accommodate site-specific N-glycan processing and we identified phosphorylated glycan structures of the mannose-6-phosphate pathway responsible for the lysosomal sorting of the glycoprotein. Importantly, our model protein can take different routes of the cellular secretory pathway, resulting in an increased glycan complexity of the secreted protein. |
| first_indexed | 2024-04-11T07:13:29Z |
| format | Article |
| id | doaj.art-88ba732ce9b345d58c74d83cbcaf2c2e |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-11T07:13:29Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-88ba732ce9b345d58c74d83cbcaf2c2e2022-12-22T04:38:04ZengElsevieriScience2589-00422022-11-012511105417Glycosylation network mapping and site-specific glycan maturation in vivoMarie-Estelle Losfeld0Ernesto Scibona1Chia-wei Lin2Markus Aebi3Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology, ETH Zürich, CH8093 Zürich, SwitzerlandInstitute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, ETH Zürich, CH8093 Zürich, SwitzerlandInstitute of Microbiology, Department of Biology, Swiss Federal Institute of Technology, ETH Zürich, CH8093 Zürich, Switzerland; Functional Genomic Center Zürich, CH8057 Zürich, SwitzerlandInstitute of Microbiology, Department of Biology, Swiss Federal Institute of Technology, ETH Zürich, CH8093 Zürich, Switzerland; Corresponding authorSummary: Glycoprotein processing along a complex highly compartmentalized pathway is a hallmark of eukaryotic cells. We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretory pathway that describes a complex set of processing reactions localized in defined intracellular compartments such as the endoplasmic reticulum the Golgi, or the lysosome. The model was able to accommodate site-specific N-glycan processing and we identified phosphorylated glycan structures of the mannose-6-phosphate pathway responsible for the lysosomal sorting of the glycoprotein. Importantly, our model protein can take different routes of the cellular secretory pathway, resulting in an increased glycan complexity of the secreted protein.http://www.sciencedirect.com/science/article/pii/S2589004222016893Cell biologyIntegrative aspects of cell biologyMathematical biosciences |
| spellingShingle | Marie-Estelle Losfeld Ernesto Scibona Chia-wei Lin Markus Aebi Glycosylation network mapping and site-specific glycan maturation in vivo iScience Cell biology Integrative aspects of cell biology Mathematical biosciences |
| title | Glycosylation network mapping and site-specific glycan maturation in vivo |
| title_full | Glycosylation network mapping and site-specific glycan maturation in vivo |
| title_fullStr | Glycosylation network mapping and site-specific glycan maturation in vivo |
| title_full_unstemmed | Glycosylation network mapping and site-specific glycan maturation in vivo |
| title_short | Glycosylation network mapping and site-specific glycan maturation in vivo |
| title_sort | glycosylation network mapping and site specific glycan maturation in vivo |
| topic | Cell biology Integrative aspects of cell biology Mathematical biosciences |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222016893 |
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