Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences
Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplific...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2008-09-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112910 |