Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays

Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays

ELISPOT, ELISA and flow cytometry techniques are often used to study the function of immune system cells. It is tempting to speculate that these assays can be used interchangeably, providing similar information about the cytokine secreting activity of cells: the higher the number of cytokine-positiv...

Full description

Bibliographic Details
Main Authors: Jodi Hagen, Ryan Zimmerman, Christine Goetz, Jody Bonnevier, Jeffrey P. Houchins, Kevin Reagan, Alexander E. Kalyuzhny
Format: Article
Language:English
Published: MDPI AG 2015-02-01
Series:Cells
Subjects:
human IFNγ
ELISPOT
ELISA
flow cytometry
human peripheral blood mononuclear cells
PBMCs
side-by-side comparison
multi-donor study
Online Access:http://www.mdpi.com/2073-4409/4/1/84

Internet

http://www.mdpi.com/2073-4409/4/1/84

Similar Items