A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation

Abstract Background Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred lo...

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Bibliographic Details
Main Authors: Kotaro Mori, Kaho Fukui, Ryotaro Amatsu, Shu Ishikawa, Valeria Verrone, Anil Wipat, Wilfried J. J. Meijer, Ken-ichi Yoshida
Format: Article
Language:English
Published: BMC 2022-03-01
Series:Microbial Cell Factories
Subjects:
Online Access:https://doi.org/10.1186/s12934-022-01759-8