Enhanced Soluble Expression of Linoleic Acid Isomerase by Coordinated Regulation of Promoter and Fusion Tag in <i>Escherichia coli</i>

Enhanced Soluble Expression of Linoleic Acid Isomerase by Coordinated Regulation of Promoter and Fusion Tag in <i>Escherichia coli</i>

PAI is a linoleic acid isomerase from <i>Propionibacterium acnes</i> and is the key enzyme in the synthesis of <i>trans</i>10, <i>cis</i>12-conjugated linoleic acid. However, the majority of the expressed PAI in <i>Escherichia coli</i> occurs in its no...

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Bibliographic Details
Main Authors: Baixi Zhang, Tong Zhu, Xintian Huang
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Foods
Subjects:
PAI
promoter
fusion tag
soluble expression
enzyme activity
Online Access:https://www.mdpi.com/2304-8158/11/10/1515

Internet

https://www.mdpi.com/2304-8158/11/10/1515

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