Protocol for nuclear dissociation of the adult C. elegans for single-nucleus RNA sequencing and its application for mapping environmental responses

Protocol for nuclear dissociation of the adult C. elegans for single-nucleus RNA sequencing and its application for mapping environmental responses

Summary: Caenorhabditis elegans is a valuable model to study organ, tissue, and cell-type responses to external cues. However, the nematode comprises multiple syncytial tissues with spatial coordinates corresponding to distinct nuclear transcriptomes. Here, we present a single-nucleus RNA sequencing...

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Bibliographic Details
Main Authors: Max T. Levenson, Rio Barrere-Cain, Lisa Truong, Yen-Wei Chen, Karissa Shuck, Blake Panter, Ella Reich, Xia Yang, Patrick Allard
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Single Cell
RNA-seq
Model Organisms
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007232

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http://www.sciencedirect.com/science/article/pii/S2666166723007232

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