Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample Analysis
Inbred strain 13/N guinea pigs are used as small animal models for the study of hemorrhagic fever viruses. Coagulation abnormalities, including prolonged clotting times and bleeding, are characteristic of hemorrhagic fever in humans; patients often meet criteria for disseminated intravascular coagul...
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MDPI AG
2020-07-01
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author | Jillian A. Condrey Timothy Flietstra Kaitlyn M. Nestor Elizabeth L. Schlosser JoAnn D. Coleman-McCray Sarah C. Genzer Stephen R. Welch Jessica R. Spengler |
author_facet | Jillian A. Condrey Timothy Flietstra Kaitlyn M. Nestor Elizabeth L. Schlosser JoAnn D. Coleman-McCray Sarah C. Genzer Stephen R. Welch Jessica R. Spengler |
author_sort | Jillian A. Condrey |
collection | DOAJ |
description | Inbred strain 13/N guinea pigs are used as small animal models for the study of hemorrhagic fever viruses. Coagulation abnormalities, including prolonged clotting times and bleeding, are characteristic of hemorrhagic fever in humans; patients often meet criteria for disseminated intravascular coagulation (DIC). Comprehensively evaluating coagulation function is critical in model development and studies of viral pathogenesis and therapeutic efficacy. Here, using the VetScan VS<i>pro</i> veterinary point-of-care platform, we developed reference intervals in both juvenile and adult strain 13/N guinea pigs for three coagulation parameters: prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen. In addition, for situations or species with limited availability of blood for clinical analysis, we investigated the validity of a modified collection approach for low-volume (0.1 mL) blood sample analysis of PT and aPTT. |
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spelling | doaj.art-892a54c1670f4e03ba0f9d6f1699e6432023-11-20T08:04:08ZengMDPI AGMicroorganisms2076-26072020-07-0188112710.3390/microorganisms8081127Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample AnalysisJillian A. Condrey0Timothy Flietstra1Kaitlyn M. Nestor2Elizabeth L. Schlosser3JoAnn D. Coleman-McCray4Sarah C. Genzer5Stephen R. Welch6Jessica R. Spengler7Comparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAComparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAComparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAComparative Medicine Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAViral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAInbred strain 13/N guinea pigs are used as small animal models for the study of hemorrhagic fever viruses. Coagulation abnormalities, including prolonged clotting times and bleeding, are characteristic of hemorrhagic fever in humans; patients often meet criteria for disseminated intravascular coagulation (DIC). Comprehensively evaluating coagulation function is critical in model development and studies of viral pathogenesis and therapeutic efficacy. Here, using the VetScan VS<i>pro</i> veterinary point-of-care platform, we developed reference intervals in both juvenile and adult strain 13/N guinea pigs for three coagulation parameters: prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen. In addition, for situations or species with limited availability of blood for clinical analysis, we investigated the validity of a modified collection approach for low-volume (0.1 mL) blood sample analysis of PT and aPTT.https://www.mdpi.com/2076-2607/8/8/1127coagulationclottinghemorrhagic feveranimal modelguinea pigstrain 13/N |
spellingShingle | Jillian A. Condrey Timothy Flietstra Kaitlyn M. Nestor Elizabeth L. Schlosser JoAnn D. Coleman-McCray Sarah C. Genzer Stephen R. Welch Jessica R. Spengler Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample Analysis Microorganisms coagulation clotting hemorrhagic fever animal model guinea pig strain 13/N |
title | Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample Analysis |
title_full | Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample Analysis |
title_fullStr | Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample Analysis |
title_full_unstemmed | Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample Analysis |
title_short | Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Reference Intervals for Inbred Strain 13/N Guinea Pigs (<i>Cavia porcellus</i>) and Validation of Low Volume Sample Analysis |
title_sort | prothrombin time activated partial thromboplastin time and fibrinogen reference intervals for inbred strain 13 n guinea pigs i cavia porcellus i and validation of low volume sample analysis |
topic | coagulation clotting hemorrhagic fever animal model guinea pig strain 13/N |
url | https://www.mdpi.com/2076-2607/8/8/1127 |
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