N-Ethyl-N-Nitrosourea (ENU) Mutagenesis Reveals an Intronic Residue Critical for Caenorhabditis elegans 3′ Splice Site Function in Vivo

N-Ethyl-N-Nitrosourea (ENU) Mutagenesis Reveals an Intronic Residue Critical for Caenorhabditis elegans 3′ Splice Site Function in Vivo

Metazoan introns contain a polypyrimidine tract immediately upstream of the AG dinucleotide that defines the 3′ splice site. In the nematode Caenorhabditis elegans, 3′ splice sites are characterized by a highly conserved UUUUCAG/R octamer motif. While the conservation of pyrimidines in this motif is...

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Bibliographic Details
Main Authors: Omar A. Itani, Stephane Flibotte, Kathleen J. Dumas, Chunfang Guo, Thomas Blumenthal, Patrick J. Hu
Format: Article
Language:English
Published: Oxford University Press 2016-06-01
Series:G3: Genes, Genomes, Genetics
Subjects:
C. elegans
splicing
3′ splice site
polypyrimidine tract
daf-12
ENU
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.116.028662

Internet

http://g3journal.org/lookup/doi/10.1534/g3.116.028662

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