Monitoring and assessment of lysosomal membrane damage in cultured cells using the high-content imager

Summary: Autophagy is an intracellular self-degradation process in which part of the cytoplasm, aggregates, or damaged organelles are degraded in lysosomes. Lysophagy is a specific form of selective autophagy responsible for clearing damaged lysosomes. Here, we present a protocol for inducing lysoso...

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Bibliographic Details
Main Authors: Keisuke Tabata, Marika Saeki, Tamotsu Yoshimori, Maho Hamasaki
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001946