Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection

Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection

Cpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet, or even surpass, Cas9 targeting rates (approaching 100% targeting), but require higher concentrations of mRNA and guide. We also demonstrate that coinjecting two gu...

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Bibliographic Details
Main Authors: Dawn E. Watkins-Chow, Gaurav K. Varshney, Lisa J. Garrett, Zelin Chen, Erin A. Jimenez, Cecilia Rivas, Kevin S. Bishop, Raman Sood, Ursula L. Harper, William J. Pavan, Shawn M. Burgess
Format: Article
Language:English
Published: Oxford University Press 2017-02-01
Series:G3: Genes, Genomes, Genetics
Subjects:
Cpf1 nuclease
genome editing
CRISPR
mouse
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.116.038091

Internet

http://g3journal.org/lookup/doi/10.1534/g3.116.038091

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