A standardized quantitative analysis strategy for stable isotope probing metagenomics

A standardized quantitative analysis strategy for stable isotope probing metagenomics

ABSTRACT Stable isotope probing (SIP) facilitates culture-independent identification of active microbial populations within complex ecosystems through isotopic enrichment of nucleic acids. Many DNA-SIP studies rely on 16S rRNA gene sequences to identify active taxa, but connecting these sequences to...

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Bibliographic Details
Main Authors: Dariia Vyshenska, Pranav Sampara, Kanwar Singh, Andy Tomatsu, W. Berkeley Kauffman, Erin E. Nuccio, Steven J. Blazewicz, Jennifer Pett-Ridge, Katherine B. Louie, Neha Varghese, Matthew Kellom, Alicia Clum, Robert Riley, Simon Roux, Emiley A. Eloe-Fadrosh, Ryan M. Ziels, Rex R. Malmstrom
Format: Article
Language:English
Published: American Society for Microbiology 2023-08-01
Series:mSystems
Subjects:
stable isotope probing
metagenomics
DNA-SIP
co-assembly
internal standards
spike-ins
Online Access:https://journals.asm.org/doi/10.1128/msystems.01280-22

Internet

https://journals.asm.org/doi/10.1128/msystems.01280-22

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