Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation

Endocytosis in neuronal dendrites is known to play a critical role in synaptic transmission and plasticity such as long-term depression (LTD). However, the inability to detect endocytosis directly in living neurons has hampered studies of its dynamics and regulation. Here, we visualized the formatio...

Full description

Bibliographic Details
Main Authors: Morgane Rosendale, Damien Jullié, Daniel Choquet, David Perrais
Format: Article
Language:English
Published: Elsevier 2017-02-01
Series:Cell Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717301663
_version_ 1818301746391285760
author Morgane Rosendale
Damien Jullié
Daniel Choquet
David Perrais
author_facet Morgane Rosendale
Damien Jullié
Daniel Choquet
David Perrais
author_sort Morgane Rosendale
collection DOAJ
description Endocytosis in neuronal dendrites is known to play a critical role in synaptic transmission and plasticity such as long-term depression (LTD). However, the inability to detect endocytosis directly in living neurons has hampered studies of its dynamics and regulation. Here, we visualized the formation of individual endocytic vesicles containing pHluorin-tagged receptors with high temporal resolution in the dendrites of cultured hippocampal neurons. We show that transferrin receptors (TfRs) are constitutively internalized at optically static clathrin-coated structures. These structures are slightly enriched near synapses that represent preferential sites for the endocytosis of postsynaptic AMPA-type receptors (AMPARs), but not for non-synaptic TfRs. Moreover, the frequency of AMPAR endocytosis events increases after the induction of NMDAR-dependent chemical LTD, but the activity of perisynaptic endocytic zones is not differentially regulated. We conclude that endocytosis is a highly dynamic and stereotyped process that internalizes receptors in precisely localized endocytic zones.
first_indexed 2024-12-13T05:27:54Z
format Article
id doaj.art-897302b0ff9646159f3857f52c4973c0
institution Directory Open Access Journal
issn 2211-1247
language English
last_indexed 2024-12-13T05:27:54Z
publishDate 2017-02-01
publisher Elsevier
record_format Article
series Cell Reports
spelling doaj.art-897302b0ff9646159f3857f52c4973c02022-12-21T23:58:08ZengElsevierCell Reports2211-12472017-02-011881840184710.1016/j.celrep.2017.01.081Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle FormationMorgane Rosendale0Damien Jullié1Daniel Choquet2David Perrais3University of Bordeaux, 33000 Bordeaux, FranceUniversity of Bordeaux, 33000 Bordeaux, FranceUniversity of Bordeaux, 33000 Bordeaux, FranceUniversity of Bordeaux, 33000 Bordeaux, FranceEndocytosis in neuronal dendrites is known to play a critical role in synaptic transmission and plasticity such as long-term depression (LTD). However, the inability to detect endocytosis directly in living neurons has hampered studies of its dynamics and regulation. Here, we visualized the formation of individual endocytic vesicles containing pHluorin-tagged receptors with high temporal resolution in the dendrites of cultured hippocampal neurons. We show that transferrin receptors (TfRs) are constitutively internalized at optically static clathrin-coated structures. These structures are slightly enriched near synapses that represent preferential sites for the endocytosis of postsynaptic AMPA-type receptors (AMPARs), but not for non-synaptic TfRs. Moreover, the frequency of AMPAR endocytosis events increases after the induction of NMDAR-dependent chemical LTD, but the activity of perisynaptic endocytic zones is not differentially regulated. We conclude that endocytosis is a highly dynamic and stereotyped process that internalizes receptors in precisely localized endocytic zones.http://www.sciencedirect.com/science/article/pii/S2211124717301663clathrin-mediated endocytosislong-term depressiontransferrin receptorBeta2 adrenergic receptorAMPA receptorGluA1GluA2post-synaptic density
spellingShingle Morgane Rosendale
Damien Jullié
Daniel Choquet
David Perrais
Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
Cell Reports
clathrin-mediated endocytosis
long-term depression
transferrin receptor
Beta2 adrenergic receptor
AMPA receptor
GluA1
GluA2
post-synaptic density
title Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
title_full Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
title_fullStr Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
title_full_unstemmed Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
title_short Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
title_sort spatial and temporal regulation of receptor endocytosis in neuronal dendrites revealed by imaging of single vesicle formation
topic clathrin-mediated endocytosis
long-term depression
transferrin receptor
Beta2 adrenergic receptor
AMPA receptor
GluA1
GluA2
post-synaptic density
url http://www.sciencedirect.com/science/article/pii/S2211124717301663
work_keys_str_mv AT morganerosendale spatialandtemporalregulationofreceptorendocytosisinneuronaldendritesrevealedbyimagingofsinglevesicleformation
AT damienjullie spatialandtemporalregulationofreceptorendocytosisinneuronaldendritesrevealedbyimagingofsinglevesicleformation
AT danielchoquet spatialandtemporalregulationofreceptorendocytosisinneuronaldendritesrevealedbyimagingofsinglevesicleformation
AT davidperrais spatialandtemporalregulationofreceptorendocytosisinneuronaldendritesrevealedbyimagingofsinglevesicleformation