Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
Endocytosis in neuronal dendrites is known to play a critical role in synaptic transmission and plasticity such as long-term depression (LTD). However, the inability to detect endocytosis directly in living neurons has hampered studies of its dynamics and regulation. Here, we visualized the formatio...
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Format: | Article |
Language: | English |
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Elsevier
2017-02-01
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Series: | Cell Reports |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717301663 |
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author | Morgane Rosendale Damien Jullié Daniel Choquet David Perrais |
author_facet | Morgane Rosendale Damien Jullié Daniel Choquet David Perrais |
author_sort | Morgane Rosendale |
collection | DOAJ |
description | Endocytosis in neuronal dendrites is known to play a critical role in synaptic transmission and plasticity such as long-term depression (LTD). However, the inability to detect endocytosis directly in living neurons has hampered studies of its dynamics and regulation. Here, we visualized the formation of individual endocytic vesicles containing pHluorin-tagged receptors with high temporal resolution in the dendrites of cultured hippocampal neurons. We show that transferrin receptors (TfRs) are constitutively internalized at optically static clathrin-coated structures. These structures are slightly enriched near synapses that represent preferential sites for the endocytosis of postsynaptic AMPA-type receptors (AMPARs), but not for non-synaptic TfRs. Moreover, the frequency of AMPAR endocytosis events increases after the induction of NMDAR-dependent chemical LTD, but the activity of perisynaptic endocytic zones is not differentially regulated. We conclude that endocytosis is a highly dynamic and stereotyped process that internalizes receptors in precisely localized endocytic zones. |
first_indexed | 2024-12-13T05:27:54Z |
format | Article |
id | doaj.art-897302b0ff9646159f3857f52c4973c0 |
institution | Directory Open Access Journal |
issn | 2211-1247 |
language | English |
last_indexed | 2024-12-13T05:27:54Z |
publishDate | 2017-02-01 |
publisher | Elsevier |
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series | Cell Reports |
spelling | doaj.art-897302b0ff9646159f3857f52c4973c02022-12-21T23:58:08ZengElsevierCell Reports2211-12472017-02-011881840184710.1016/j.celrep.2017.01.081Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle FormationMorgane Rosendale0Damien Jullié1Daniel Choquet2David Perrais3University of Bordeaux, 33000 Bordeaux, FranceUniversity of Bordeaux, 33000 Bordeaux, FranceUniversity of Bordeaux, 33000 Bordeaux, FranceUniversity of Bordeaux, 33000 Bordeaux, FranceEndocytosis in neuronal dendrites is known to play a critical role in synaptic transmission and plasticity such as long-term depression (LTD). However, the inability to detect endocytosis directly in living neurons has hampered studies of its dynamics and regulation. Here, we visualized the formation of individual endocytic vesicles containing pHluorin-tagged receptors with high temporal resolution in the dendrites of cultured hippocampal neurons. We show that transferrin receptors (TfRs) are constitutively internalized at optically static clathrin-coated structures. These structures are slightly enriched near synapses that represent preferential sites for the endocytosis of postsynaptic AMPA-type receptors (AMPARs), but not for non-synaptic TfRs. Moreover, the frequency of AMPAR endocytosis events increases after the induction of NMDAR-dependent chemical LTD, but the activity of perisynaptic endocytic zones is not differentially regulated. We conclude that endocytosis is a highly dynamic and stereotyped process that internalizes receptors in precisely localized endocytic zones.http://www.sciencedirect.com/science/article/pii/S2211124717301663clathrin-mediated endocytosislong-term depressiontransferrin receptorBeta2 adrenergic receptorAMPA receptorGluA1GluA2post-synaptic density |
spellingShingle | Morgane Rosendale Damien Jullié Daniel Choquet David Perrais Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation Cell Reports clathrin-mediated endocytosis long-term depression transferrin receptor Beta2 adrenergic receptor AMPA receptor GluA1 GluA2 post-synaptic density |
title | Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation |
title_full | Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation |
title_fullStr | Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation |
title_full_unstemmed | Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation |
title_short | Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation |
title_sort | spatial and temporal regulation of receptor endocytosis in neuronal dendrites revealed by imaging of single vesicle formation |
topic | clathrin-mediated endocytosis long-term depression transferrin receptor Beta2 adrenergic receptor AMPA receptor GluA1 GluA2 post-synaptic density |
url | http://www.sciencedirect.com/science/article/pii/S2211124717301663 |
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