A Split-Ubiquitin Based Strategy Selecting for Protein Complex-Interfering Mutations
Understanding the topologies and functions of protein interaction networks requires the selective removal of single interactions. We introduce a selection strategy that enriches among a random library of alleles for mutations that impair the binding to a given partner protein. The selection makes us...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2016-09-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.116.031369 |