Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis Patients
Background: Matrix metalloproteinases (MMPs) are pleiotropic enzymes involved in extracellular protein degradation and turnover. MMPs are implicated in the pathogenesis of many neurological diseases, including multiple sclerosis (MS).Objective: To search the level of MMPs in the cerebrospinal fluid...
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| Format: | Article |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fneur.2018.01080/full |
| _version_ | 1811204676685660160 |
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| author | Massimiliano Castellazzi Massimiliano Castellazzi Daniela Ligi Elena Contaldi Davide Quartana Mattia Fonderico Luca Borgatti Tiziana Bellini Tiziana Bellini Tiziana Bellini Alessandro Trentini Enrico Granieri Enrico Fainardi Enrico Fainardi Ferdinando Mannello Maura Pugliatti Maura Pugliatti |
| author_facet | Massimiliano Castellazzi Massimiliano Castellazzi Daniela Ligi Elena Contaldi Davide Quartana Mattia Fonderico Luca Borgatti Tiziana Bellini Tiziana Bellini Tiziana Bellini Alessandro Trentini Enrico Granieri Enrico Fainardi Enrico Fainardi Ferdinando Mannello Maura Pugliatti Maura Pugliatti |
| author_sort | Massimiliano Castellazzi |
| collection | DOAJ |
| description | Background: Matrix metalloproteinases (MMPs) are pleiotropic enzymes involved in extracellular protein degradation and turnover. MMPs are implicated in the pathogenesis of many neurological diseases, including multiple sclerosis (MS).Objective: To search the level of MMPs in the cerebrospinal fluid (CSF) of MS patients and detect possible disease-specific patterns.Methods: CSF samples from 32 MS patients and, from 15 control subjects with other inflammatory neurological diseases (OIND) were analyzed. The Bio-Plex Pro Human MMP 9-Plex Panel (Bio-Rad) was used for the quantification of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, and MMP-13.Results: CSF MMP-1 and MMP-12 levels were significantly reduced in MS as compared with OIND. In MS patients' CSF: (i) MMP-1 levels were significantly higher in women vs. men; (ii) MMP-10 concentrations were higher in patients with CSF-restricted IgG oligoclonal bands, and (iii) MMP-7 levels were increased in patients with longer disease duration. In the OIND group MMP-7 and MMP-12 levels significantly and directly correlated with age.Conclusions: Our study contributes to investigating the role of MMPs in MS, with regard to CSF immunological features and disease duration. Sex-specific differences were also detected in MMPs CSF levels. |
| first_indexed | 2024-04-12T03:16:34Z |
| format | Article |
| id | doaj.art-89991f52ec714c07a72ffdad0d3508e1 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-04-12T03:16:34Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-89991f52ec714c07a72ffdad0d3508e12022-12-22T03:50:04ZengFrontiers Media S.A.Frontiers in Neurology1664-22952018-12-01910.3389/fneur.2018.01080421664Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis PatientsMassimiliano Castellazzi0Massimiliano Castellazzi1Daniela Ligi2Elena Contaldi3Davide Quartana4Mattia Fonderico5Luca Borgatti6Tiziana Bellini7Tiziana Bellini8Tiziana Bellini9Alessandro Trentini10Enrico Granieri11Enrico Fainardi12Enrico Fainardi13Ferdinando Mannello14Maura Pugliatti15Maura Pugliatti16Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyInterdepartmental Research Center for the Study of Multiple Sclerosis and Inflammatory and Degenerative Diseases of the Nervous System, University of Ferrara, Ferrara, ItalyDepartment of Biomolecular Sciences, University “Carlo Bo” of Urbino, Urbino, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalySchool of Medicine, University of Ferrara, Ferrara, ItalyDepartment of Neuroscience and Rehabilitation, Azienda Ospedaliero-Universitaria di Ferrara, Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyInterdepartmental Research Center for the Study of Multiple Sclerosis and Inflammatory and Degenerative Diseases of the Nervous System, University of Ferrara, Ferrara, ItalyUniversity Center for Studies on Gender Medicine, University of Ferrara, Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyInterdepartmental Research Center for the Study of Multiple Sclerosis and Inflammatory and Degenerative Diseases of the Nervous System, University of Ferrara, Ferrara, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, ItalyDepartment of Biomolecular Sciences, University “Carlo Bo” of Urbino, Urbino, ItalyDepartment of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, ItalyInterdepartmental Research Center for the Study of Multiple Sclerosis and Inflammatory and Degenerative Diseases of the Nervous System, University of Ferrara, Ferrara, ItalyBackground: Matrix metalloproteinases (MMPs) are pleiotropic enzymes involved in extracellular protein degradation and turnover. MMPs are implicated in the pathogenesis of many neurological diseases, including multiple sclerosis (MS).Objective: To search the level of MMPs in the cerebrospinal fluid (CSF) of MS patients and detect possible disease-specific patterns.Methods: CSF samples from 32 MS patients and, from 15 control subjects with other inflammatory neurological diseases (OIND) were analyzed. The Bio-Plex Pro Human MMP 9-Plex Panel (Bio-Rad) was used for the quantification of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, and MMP-13.Results: CSF MMP-1 and MMP-12 levels were significantly reduced in MS as compared with OIND. In MS patients' CSF: (i) MMP-1 levels were significantly higher in women vs. men; (ii) MMP-10 concentrations were higher in patients with CSF-restricted IgG oligoclonal bands, and (iii) MMP-7 levels were increased in patients with longer disease duration. In the OIND group MMP-7 and MMP-12 levels significantly and directly correlated with age.Conclusions: Our study contributes to investigating the role of MMPs in MS, with regard to CSF immunological features and disease duration. Sex-specific differences were also detected in MMPs CSF levels.https://www.frontiersin.org/article/10.3389/fneur.2018.01080/fullmultiple sclerosiscerebrospinal fluidmatrix metalloproteinasessexagingblood-brain-barrier |
| spellingShingle | Massimiliano Castellazzi Massimiliano Castellazzi Daniela Ligi Elena Contaldi Davide Quartana Mattia Fonderico Luca Borgatti Tiziana Bellini Tiziana Bellini Tiziana Bellini Alessandro Trentini Enrico Granieri Enrico Fainardi Enrico Fainardi Ferdinando Mannello Maura Pugliatti Maura Pugliatti Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis Patients Frontiers in Neurology multiple sclerosis cerebrospinal fluid matrix metalloproteinases sex aging blood-brain-barrier |
| title | Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis Patients |
| title_full | Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis Patients |
| title_fullStr | Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis Patients |
| title_full_unstemmed | Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis Patients |
| title_short | Multiplex Matrix Metalloproteinases Analysis in the Cerebrospinal Fluid Reveals Potential Specific Patterns in Multiple Sclerosis Patients |
| title_sort | multiplex matrix metalloproteinases analysis in the cerebrospinal fluid reveals potential specific patterns in multiple sclerosis patients |
| topic | multiple sclerosis cerebrospinal fluid matrix metalloproteinases sex aging blood-brain-barrier |
| url | https://www.frontiersin.org/article/10.3389/fneur.2018.01080/full |
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