Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis

Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis

Abstract Background Cotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered the development of cotton functional genomics. The protoplast system has proven to be an important and versatile tool for functional genomics,...

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Bibliographic Details
Main Authors: Ke Zhang, Shanhe Liu, Yunze Fu, Zixuan Wang, Xiubo Yang, Wenjing Li, Caihua Zhang, Dongmei Zhang, Jun Li
Format: Article
Language:English
Published: BMC 2023-01-01
Series:Plant Methods
Subjects:
Protoplast isolation
PEG-mediated transfection
scRNA-seq
Genome editing
Cotton
Online Access:https://doi.org/10.1186/s13007-023-00983-6

Internet

https://doi.org/10.1186/s13007-023-00983-6

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