A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
<p>Abstract</p> <p>Background</p> <p>The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences. Uracil-excision based cloning is ligase and sequence independent and...
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| Format: | Article |
| Language: | English |
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BMC
2010-03-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/10/21 |