Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats
Attenuated skeletal muscle glucose uptake (GU) has been observed with advancing age. It is important to elucidate the mechanisms linked to interventions that oppose this detrimental outcome. Earlier research using young rodents and (or) cultured myocytes reported that treatment with 5-aminoimidazole...
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| Format: | Article |
| Language: | English |
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Canadian Science Publishing
2022-01-01
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| Series: | FACETS |
| Subjects: | |
| Online Access: | https://facetsjournal.com/doi/10.1139/facets-2021-0166 |
| _version_ | 1811232738839101440 |
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| author | Haiyan Wang Amy Zheng Edward B. Arias Gregory D. Cartee |
| author_facet | Haiyan Wang Amy Zheng Edward B. Arias Gregory D. Cartee |
| author_sort | Haiyan Wang |
| collection | DOAJ |
| description | Attenuated skeletal muscle glucose uptake (GU) has been observed with advancing age. It is important to elucidate the mechanisms linked to interventions that oppose this detrimental outcome. Earlier research using young rodents and (or) cultured myocytes reported that treatment with 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR; an AMP-activated protein kinase (AMPK) activator) can increase γ3-AMPK activity and reduce membrane cholesterol content, each of which has been proposed to elevate GU. However, the effect of AICAR treatment on γ3-AMPK activity and membrane cholesterol in skeletal muscle of aged animals has not been reported. Our purpose was to evaluate the effects of AICAR treatment on these potential mechanisms for enhanced glucose uptake in the skeletal muscle of aged animals. Epitrochlearis muscles from 26–27-month-old male rats were isolated and incubated ± AICAR, followed by 3 h incubation without AICAR, and then incubation with 3-O-methyl-[3 H] glucose (to assess GU ± insulin). Muscles were also analyzed for γ3-AMPK activity and membrane cholesterol content. Prior AICAR treatment led to increased γ3-AMPK activity, reduced membrane cholesterol content, and enhanced glucose uptake in skeletal muscle from aged rats. These observations revealed that two potential mechanisms for greater GU previously observed in younger animals and (or) cell models are also potentially relevant for enhanced GU by muscles from older animals. |
| first_indexed | 2024-04-12T11:07:59Z |
| format | Article |
| id | doaj.art-89b4363a2a87439985c6550f04f9160c |
| institution | Directory Open Access Journal |
| issn | 2371-1671 |
| language | English |
| last_indexed | 2024-04-12T11:07:59Z |
| publishDate | 2022-01-01 |
| publisher | Canadian Science Publishing |
| record_format | Article |
| series | FACETS |
| spelling | doaj.art-89b4363a2a87439985c6550f04f9160c2022-12-22T03:35:41ZengCanadian Science PublishingFACETS2371-16712022-01-01777479110.1139/facets-2021-0166Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old ratsHaiyan Wang0Amy Zheng1Edward B. Arias2Gregory D. Cartee3Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, USAMuscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, USAMuscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, USAMuscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, USAAttenuated skeletal muscle glucose uptake (GU) has been observed with advancing age. It is important to elucidate the mechanisms linked to interventions that oppose this detrimental outcome. Earlier research using young rodents and (or) cultured myocytes reported that treatment with 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR; an AMP-activated protein kinase (AMPK) activator) can increase γ3-AMPK activity and reduce membrane cholesterol content, each of which has been proposed to elevate GU. However, the effect of AICAR treatment on γ3-AMPK activity and membrane cholesterol in skeletal muscle of aged animals has not been reported. Our purpose was to evaluate the effects of AICAR treatment on these potential mechanisms for enhanced glucose uptake in the skeletal muscle of aged animals. Epitrochlearis muscles from 26–27-month-old male rats were isolated and incubated ± AICAR, followed by 3 h incubation without AICAR, and then incubation with 3-O-methyl-[3 H] glucose (to assess GU ± insulin). Muscles were also analyzed for γ3-AMPK activity and membrane cholesterol content. Prior AICAR treatment led to increased γ3-AMPK activity, reduced membrane cholesterol content, and enhanced glucose uptake in skeletal muscle from aged rats. These observations revealed that two potential mechanisms for greater GU previously observed in younger animals and (or) cell models are also potentially relevant for enhanced GU by muscles from older animals.https://facetsjournal.com/doi/10.1139/facets-2021-0166insulin sensitivityglucose transportAMP-activated protein kinaseagingcholesterolskeletal muscle |
| spellingShingle | Haiyan Wang Amy Zheng Edward B. Arias Gregory D. Cartee Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats FACETS insulin sensitivity glucose transport AMP-activated protein kinase aging cholesterol skeletal muscle |
| title | Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats |
| title_full | Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats |
| title_fullStr | Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats |
| title_full_unstemmed | Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats |
| title_short | Prior AICAR induces elevated glucose uptake concomitant with greater γ3-AMPK activation and reduced membrane cholesterol in skeletal muscle from 26-month-old rats |
| title_sort | prior aicar induces elevated glucose uptake concomitant with greater γ3 ampk activation and reduced membrane cholesterol in skeletal muscle from 26 month old rats |
| topic | insulin sensitivity glucose transport AMP-activated protein kinase aging cholesterol skeletal muscle |
| url | https://facetsjournal.com/doi/10.1139/facets-2021-0166 |
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