Enhancing the throughput and multiplexing capabilities of next generation sequencing for efficient implementation of pooled shRNA and CRISPR screens

Enhancing the throughput and multiplexing capabilities of next generation sequencing for efficient implementation of pooled shRNA and CRISPR screens

Abstract Next generation sequencing is becoming the method of choice for functional genomic studies that use pooled shRNA or CRISPR libraries. A key challenge in sequencing these mixed-oligo libraries is that they are highly susceptible to hairpin and/or heteroduplex formation. This results in polyc...

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Bibliographic Details
Main Authors: Md. Fahmid Islam, Atsushi Watanabe, Lai Wong, Conor Lazarou, Frederick S. Vizeacoumar, Omar Abuhussein, Wayne Hill, Maruti Uppalapati, C. Ronald Geyer, Franco J. Vizeacoumar
Format: Article
Language:English
Published: Nature Portfolio 2017-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-01170-z

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https://doi.org/10.1038/s41598-017-01170-z

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