Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effect
Background: Microglia are resident immune cells of the central nervous system that sense environmental changes and maintain central nervous system homeostasis. Dysfunctional microglia produce toxic mediators that lead to neuronal death. Recent studies suggest that Sodium Aescinate has a neuroprotect...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1086429/full |
| _version_ | 1797942958995013632 |
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| author | Fei Xu Fei Xu Yiguo Jiang Xiaoyu Wang Li Shen Yan Yan Dongkai Guo Cheng Wang Cheng Wang |
| author_facet | Fei Xu Fei Xu Yiguo Jiang Xiaoyu Wang Li Shen Yan Yan Dongkai Guo Cheng Wang Cheng Wang |
| author_sort | Fei Xu |
| collection | DOAJ |
| description | Background: Microglia are resident immune cells of the central nervous system that sense environmental changes and maintain central nervous system homeostasis. Dysfunctional microglia produce toxic mediators that lead to neuronal death. Recent studies suggest that Sodium Aescinate has a neuroprotective effect. However, it is unclear whether Sodium Aescinate exerts neuroprotective effects by inhibiting activation of microglia.Method: Traumatic brain injury and lipopolysaccharide neuroinflammation model were used to evaluate the microglia activation in vivo. BV2 and primary microglia cells were used to assess the microglia activation in vitro. Molecular docking technique was used to predict the binding energy of Sodium Aescinate to NF-κB signaling pathway proteins.Result: Sodium Aescinate inhibited microglial activation in-vivo and in-vitro. Sodium Aescinate inhibited the activation of microglia in Traumatic brain injury and lipopolysaccharide mouse models. Sodium Aescinate also inhibited the expression of inflammatory proteins in BV2 and primary microglia cells. Western blot experiment showed that SA inhibited the activation of NF-κB pathway in BV2 and primary microglia cells. Molecular docking results also showed that Sodium Aescinate had a better affinity with the core protein of the NF-κB pathway. Western blot identified that SA inhibited activation of NF-κB pathway. In Traumatic brain injury model and conditioned medium experiment, Sodium Aescinate pretreatment inhibited inflammation and protected neuron.Conclusion: Our study confirmed that the protection effects of Sodium Aescinate on neurons by inhibiting microglia activation through NF-κB pathway. |
| first_indexed | 2024-04-10T20:16:41Z |
| format | Article |
| id | doaj.art-89c0e764232942498efca22179f34e49 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-10T20:16:41Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-89c0e764232942498efca22179f34e492023-01-26T05:18:31ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-01-011410.3389/fphar.2023.10864291086429Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effectFei Xu0Fei Xu1Yiguo Jiang2Xiaoyu Wang3Li Shen4Yan Yan5Dongkai Guo6Cheng Wang7Cheng Wang8Department of Pharmacy, Suzhou Science and Technology Town Hospital, Suzhou, ChinaDepartment of Pharmacy, The People’s Hospital of Suzhou New District, Suzhou, ChinaDepartment of Pharmacy, Suzhou Science and Technology Town Hospital, Suzhou, ChinaDepartment of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, ChinaDepartment of Pharmacy, Suzhou Science and Technology Town Hospital, Suzhou, ChinaDepartment of Neurology, Suzhou Science and Technology Town Hospital, Suzhou, ChinaDepartment of Pharmacy, Suzhou Science and Technology Town Hospital, Suzhou, ChinaDepartment of Pharmacy, Suzhou Science and Technology Town Hospital, Suzhou, ChinaHigh-tech Zone social utilities bureau of Suzhou, Suzhou, ChinaBackground: Microglia are resident immune cells of the central nervous system that sense environmental changes and maintain central nervous system homeostasis. Dysfunctional microglia produce toxic mediators that lead to neuronal death. Recent studies suggest that Sodium Aescinate has a neuroprotective effect. However, it is unclear whether Sodium Aescinate exerts neuroprotective effects by inhibiting activation of microglia.Method: Traumatic brain injury and lipopolysaccharide neuroinflammation model were used to evaluate the microglia activation in vivo. BV2 and primary microglia cells were used to assess the microglia activation in vitro. Molecular docking technique was used to predict the binding energy of Sodium Aescinate to NF-κB signaling pathway proteins.Result: Sodium Aescinate inhibited microglial activation in-vivo and in-vitro. Sodium Aescinate inhibited the activation of microglia in Traumatic brain injury and lipopolysaccharide mouse models. Sodium Aescinate also inhibited the expression of inflammatory proteins in BV2 and primary microglia cells. Western blot experiment showed that SA inhibited the activation of NF-κB pathway in BV2 and primary microglia cells. Molecular docking results also showed that Sodium Aescinate had a better affinity with the core protein of the NF-κB pathway. Western blot identified that SA inhibited activation of NF-κB pathway. In Traumatic brain injury model and conditioned medium experiment, Sodium Aescinate pretreatment inhibited inflammation and protected neuron.Conclusion: Our study confirmed that the protection effects of Sodium Aescinate on neurons by inhibiting microglia activation through NF-κB pathway.https://www.frontiersin.org/articles/10.3389/fphar.2023.1086429/fullsodium aescinatemicrogliaNF-κB pathwayneuroinflammationmolecular docking |
| spellingShingle | Fei Xu Fei Xu Yiguo Jiang Xiaoyu Wang Li Shen Yan Yan Dongkai Guo Cheng Wang Cheng Wang Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effect Frontiers in Pharmacology sodium aescinate microglia NF-κB pathway neuroinflammation molecular docking |
| title | Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effect |
| title_full | Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effect |
| title_fullStr | Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effect |
| title_full_unstemmed | Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effect |
| title_short | Sodium aescinate inhibits microglia activation through NF-κB pathway and exerts neuroprotective effect |
| title_sort | sodium aescinate inhibits microglia activation through nf κb pathway and exerts neuroprotective effect |
| topic | sodium aescinate microglia NF-κB pathway neuroinflammation molecular docking |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1086429/full |
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