Optimization of secreted recombinant human epidermal growth factor production using pectate lyase B from Escherichia coli BL21(DE3) by central composite design and its production in high cell density culture

Optimization of secreted recombinant human epidermal growth factor production using pectate lyase B from Escherichia coli BL21(DE3) by central composite design and its production in high cell density culture

Context: Human Epidermal Growth Factor (hEGF) is a potential therapeutic protein that has been widely used as a healing agent for various chronic wounds. It induces the proliferation and metabolism of epithelial cells, regenerates skin cells, and validates skin elasticity. In the previous study, rec...

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Bibliographic Details
Main Authors: Sriwidodo Sriwidodo, Toto Subroto, Iman P Maksum, Nasrul Wathoni, Tina Rostinawati, Himmatul Ulya, Indah U Putri
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2019-01-01
Series:Journal of Pharmacy and Bioallied Sciences
Subjects:
central composite design
escherichia coli bl21(de3)
high cell-density culture
recombinant human epidermal growth factor
Online Access:http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2019;volume=11;issue=8;spage=562;epage=566;aulast=Sriwidodo

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http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2019;volume=11;issue=8;spage=562;epage=566;aulast=Sriwidodo

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