Identification of the insertion site of transgenic DNA based on cyclization of the target gene with the flanking sequence and nested inverse PCR

Identification of the insertion site of transgenic DNA based on cyclization of the target gene with the flanking sequence and nested inverse PCR

We demonstrate a novel method for identification of the insertion site of transgenic DNA by construction a circular DNA containing part of the transgenic DNA (tDNA) and its flanking sequence, which would be different from those in the native genomic DNA (gDNA). Using follistatin (FST) as a model of...

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Bibliographic Details
Main Authors: Jiayu Wang, Xuetong Bi, Wei Chen, Qinyue Zhao, Jinqi Yang, Xiangjun Tong, Meiping Zhao
Format: Article
Language:English
Published: Elsevier 2021-08-01
Series:Talanta Open
Subjects:
Transgenic DNA
DNA bait capture
DNA cyclization
T7 DNA ligase
Nested inverse PCR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666831921000035

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http://www.sciencedirect.com/science/article/pii/S2666831921000035

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