Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1
Cohesin’s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires op...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/44736 |
| _version_ | 1828203189851127808 |
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| author | Madhusudhan Srinivasan Naomi J Petela Johanna C Scheinost James Collier Menelaos Voulgaris Maurici B Roig Frederic Beckouët Bin Hu Kim A Nasmyth |
| author_facet | Madhusudhan Srinivasan Naomi J Petela Johanna C Scheinost James Collier Menelaos Voulgaris Maurici B Roig Frederic Beckouët Bin Hu Kim A Nasmyth |
| author_sort | Madhusudhan Srinivasan |
| collection | DOAJ |
| description | Cohesin’s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1’s ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot be turned back on without cohesin’s dissociation from chromosomes. The latter phenomenon enabled us to show that in the absence of release mechanisms, cohesin rings that have already captured DNA in a Scc2-dependent manner before replication no longer require Scc2 to capture sister DNAs during S phase. |
| first_indexed | 2024-04-12T12:01:29Z |
| format | Article |
| id | doaj.art-89edaeca40b24c9ab0ebe82c857ed8a1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:01:29Z |
| publishDate | 2019-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-89edaeca40b24c9ab0ebe82c857ed8a12022-12-22T03:33:51ZengeLife Sciences Publications LtdeLife2050-084X2019-06-01810.7554/eLife.44736Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1Madhusudhan Srinivasan0https://orcid.org/0000-0001-5676-4219Naomi J Petela1Johanna C Scheinost2James Collier3Menelaos Voulgaris4Maurici B Roig5Frederic Beckouët6Bin Hu7Kim A Nasmyth8https://orcid.org/0000-0001-7030-4403Department of Biochemistry, University of Oxford, Oxford, United KingdomDepartment of Biochemistry, University of Oxford, Oxford, United KingdomDepartment of Biochemistry, University of Oxford, Oxford, United KingdomDepartment of Biochemistry, University of Oxford, Oxford, United KingdomDepartment of Biochemistry, University of Oxford, Oxford, United KingdomDepartment of Biochemistry, University of Oxford, Oxford, United KingdomLaboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative (CBI), Université de Toulouse, Toulouse, FranceDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United KingdomDepartment of Biochemistry, University of Oxford, Oxford, United KingdomCohesin’s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1’s ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot be turned back on without cohesin’s dissociation from chromosomes. The latter phenomenon enabled us to show that in the absence of release mechanisms, cohesin rings that have already captured DNA in a Scc2-dependent manner before replication no longer require Scc2 to capture sister DNAs during S phase.https://elifesciences.org/articles/44736CohesinCDK1sister chromatid cohesionloop extrusionWaplSMC |
| spellingShingle | Madhusudhan Srinivasan Naomi J Petela Johanna C Scheinost James Collier Menelaos Voulgaris Maurici B Roig Frederic Beckouët Bin Hu Kim A Nasmyth Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1 eLife Cohesin CDK1 sister chromatid cohesion loop extrusion Wapl SMC |
| title | Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1 |
| title_full | Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1 |
| title_fullStr | Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1 |
| title_full_unstemmed | Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1 |
| title_short | Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1 |
| title_sort | scc2 counteracts a wapl independent mechanism that releases cohesin from chromosomes during g1 |
| topic | Cohesin CDK1 sister chromatid cohesion loop extrusion Wapl SMC |
| url | https://elifesciences.org/articles/44736 |
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