Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system

Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system

Summary: Environmental stimuli evoke transient increases of the cytosolic Ca2+ level. To identify upstream components of Ca2+ signaling, we have optimized two forward genetic screening systems based on Ca2+ reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (...

Full description

Bibliographic Details
Main Authors: Shujing Sun, Xiaoyan Zhang, Kong Chen, Xiaohong Zhu, Yang Zhao
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Genetics
High Throughput Screening
Model Organisms
Molecular/Chemical Probes
Plant sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002653

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721002653

Similar Items