Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system
Summary: Environmental stimuli evoke transient increases of the cytosolic Ca2+ level. To identify upstream components of Ca2+ signaling, we have optimized two forward genetic screening systems based on Ca2+ reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721002653 |
| _version_ | 1819069102107394048 |
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| author | Shujing Sun Xiaoyan Zhang Kong Chen Xiaohong Zhu Yang Zhao |
| author_facet | Shujing Sun Xiaoyan Zhang Kong Chen Xiaohong Zhu Yang Zhao |
| author_sort | Shujing Sun |
| collection | DOAJ |
| description | Summary: Environmental stimuli evoke transient increases of the cytosolic Ca2+ level. To identify upstream components of Ca2+ signaling, we have optimized two forward genetic screening systems based on Ca2+ reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQsig6 EMS-mutagenized library was preferably used to screen the mutants with reduced Ca2+ signal response due to its high effectiveness, while the AEQub EMS-mutagenized library was used for screening of the mutants with altered Ca2+ signal response.For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) and Zhu et al. (2013). |
| first_indexed | 2024-12-21T16:44:42Z |
| format | Article |
| id | doaj.art-89fa68c4d6f44d7c94dd874fe9b58f20 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T16:44:42Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-89fa68c4d6f44d7c94dd874fe9b58f202022-12-21T18:57:01ZengElsevierSTAR Protocols2666-16672021-06-0122100558Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter systemShujing Sun0Xiaoyan Zhang1Kong Chen2Xiaohong Zhu3Yang Zhao4Shanghai Center for Plant Stress Biology, and CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaState Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475001, China; State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475001, ChinaShanghai Center for Plant Stress Biology, and CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475001, China; State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475001, ChinaShanghai Center for Plant Stress Biology, and CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China; State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475001, China; Corresponding authorSummary: Environmental stimuli evoke transient increases of the cytosolic Ca2+ level. To identify upstream components of Ca2+ signaling, we have optimized two forward genetic screening systems based on Ca2+ reporter aequorin. AEQsig6 and AEQub plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQsig6 EMS-mutagenized library was preferably used to screen the mutants with reduced Ca2+ signal response due to its high effectiveness, while the AEQub EMS-mutagenized library was used for screening of the mutants with altered Ca2+ signal response.For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) and Zhu et al. (2013).http://www.sciencedirect.com/science/article/pii/S2666166721002653GeneticsHigh Throughput ScreeningModel OrganismsMolecular/Chemical ProbesPlant sciences |
| spellingShingle | Shujing Sun Xiaoyan Zhang Kong Chen Xiaohong Zhu Yang Zhao Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system STAR Protocols Genetics High Throughput Screening Model Organisms Molecular/Chemical Probes Plant sciences |
| title | Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system |
| title_full | Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system |
| title_fullStr | Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system |
| title_full_unstemmed | Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system |
| title_short | Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system |
| title_sort | screening for arabidopsis mutants with altered ca2 signal response using aequorin based ca2 reporter system |
| topic | Genetics High Throughput Screening Model Organisms Molecular/Chemical Probes Plant sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721002653 |
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