Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma
Aberrant sialylation is frequently observed in tumor development, but which sialyltransferases are involved in this event are not well known. Herein, we performed comprehensive analyses on six ST3GAL family members, the α-2,3 sialyltransferases, in clear cell renal cell carcinoma (ccRCC) from public...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.979605/full |
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| author | Jiakuan Liu Jiakuan Liu Meiqian Li Meiqian Li Jiajun Wu Qi Qi Yang Li Simei Wang Simei Wang Shengjie Liang Yuqing Zhang Zhitao Zhu Ruimin Huang Ruimin Huang Jun Yan Jun Yan Rujian Zhu |
| author_facet | Jiakuan Liu Jiakuan Liu Meiqian Li Meiqian Li Jiajun Wu Qi Qi Yang Li Simei Wang Simei Wang Shengjie Liang Yuqing Zhang Zhitao Zhu Ruimin Huang Ruimin Huang Jun Yan Jun Yan Rujian Zhu |
| author_sort | Jiakuan Liu |
| collection | DOAJ |
| description | Aberrant sialylation is frequently observed in tumor development, but which sialyltransferases are involved in this event are not well known. Herein, we performed comprehensive analyses on six ST3GAL family members, the α-2,3 sialyltransferases, in clear cell renal cell carcinoma (ccRCC) from public datasets. Only ST3GAL5 was consistently and significantly overexpressed in ccRCC (n = 791 in total), compared with normal kidney tissues. Its overexpression was positively correlated with tumor stage, grade, and the poor prognosis in ccRCC patients. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses indicated the involvement of ST3GAL5 in tumor immunoregulation. Then we revealed that ST3GAL5 expression showed a positive correlation with CD8+ T cell infiltration, using multiple tools on TIMER2.0 web server. Notably, ST3GAL5 overexpression was further identified to be associated with expression signature of CD8+ T cell exhaustion in ccRCC samples from three datasets (n = 867 in total; r > 0.3, p < 0.001). In our own ccRCC cohort (n = 45), immunohistochemistry and immunofluorescence staining confirmed that ST3GAL5 overexpression was accompanied by high CD8+ T cell infiltration with the increased exhaustion markers. Altogether, ST3GAL5 as a promising prognostic biomarker with CD8+ T cell exhaustion in ccRCC is indicated. |
| first_indexed | 2024-04-12T04:43:36Z |
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| id | doaj.art-8a4d49da0e624fde8c1158eb974371ef |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T04:43:36Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8a4d49da0e624fde8c1158eb974371ef2022-12-22T03:47:34ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-09-011310.3389/fimmu.2022.979605979605Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinomaJiakuan Liu0Jiakuan Liu1Meiqian Li2Meiqian Li3Jiajun Wu4Qi Qi5Yang Li6Simei Wang7Simei Wang8Shengjie Liang9Yuqing Zhang10Zhitao Zhu11Ruimin Huang12Ruimin Huang13Jun Yan14Jun Yan15Rujian Zhu16Department of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaDepartment of Laboratory Animal Science, Fudan University, Shanghai, ChinaDepartment of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaModel Animal Research Center of Nanjing University, Nanjing University, Jiangsu, ChinaDepartment of Laboratory Animal Science, Fudan University, Shanghai, ChinaDepartment of Laboratory Animal Science, Fudan University, Shanghai, ChinaDepartment of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaDepartment of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaDepartment of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaDepartment of Laboratory Animal Science, Fudan University, Shanghai, ChinaShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaDepartment of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaDepartment of Laboratory Animal Science, Fudan University, Shanghai, ChinaDepartment of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaAberrant sialylation is frequently observed in tumor development, but which sialyltransferases are involved in this event are not well known. Herein, we performed comprehensive analyses on six ST3GAL family members, the α-2,3 sialyltransferases, in clear cell renal cell carcinoma (ccRCC) from public datasets. Only ST3GAL5 was consistently and significantly overexpressed in ccRCC (n = 791 in total), compared with normal kidney tissues. Its overexpression was positively correlated with tumor stage, grade, and the poor prognosis in ccRCC patients. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses indicated the involvement of ST3GAL5 in tumor immunoregulation. Then we revealed that ST3GAL5 expression showed a positive correlation with CD8+ T cell infiltration, using multiple tools on TIMER2.0 web server. Notably, ST3GAL5 overexpression was further identified to be associated with expression signature of CD8+ T cell exhaustion in ccRCC samples from three datasets (n = 867 in total; r > 0.3, p < 0.001). In our own ccRCC cohort (n = 45), immunohistochemistry and immunofluorescence staining confirmed that ST3GAL5 overexpression was accompanied by high CD8+ T cell infiltration with the increased exhaustion markers. Altogether, ST3GAL5 as a promising prognostic biomarker with CD8+ T cell exhaustion in ccRCC is indicated.https://www.frontiersin.org/articles/10.3389/fimmu.2022.979605/fullsialylationclear cell renal cell carcinomaST3GAL5CD8+ T cell exhaustionprognostic biomarker |
| spellingShingle | Jiakuan Liu Jiakuan Liu Meiqian Li Meiqian Li Jiajun Wu Qi Qi Yang Li Simei Wang Simei Wang Shengjie Liang Yuqing Zhang Zhitao Zhu Ruimin Huang Ruimin Huang Jun Yan Jun Yan Rujian Zhu Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma Frontiers in Immunology sialylation clear cell renal cell carcinoma ST3GAL5 CD8+ T cell exhaustion prognostic biomarker |
| title | Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma |
| title_full | Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma |
| title_fullStr | Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma |
| title_full_unstemmed | Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma |
| title_short | Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma |
| title_sort | identification of st3gal5 as a prognostic biomarker correlating with cd8 t cell exhaustion in clear cell renal cell carcinoma |
| topic | sialylation clear cell renal cell carcinoma ST3GAL5 CD8+ T cell exhaustion prognostic biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.979605/full |
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