Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay

Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay

Sperm DNA damage is associated with fertilization failure, impaired pre-and post- embryo implantation and poor pregnancy outcome. A series of methodologies to assess DNA damage in spermatozoa have been developed but most are unable to differentiate between single-stranded DNA breaks (SSBs) and doubl...

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Bibliographic Details
Main Authors: Elva I eCortes-Gutierrez, Carmen eLopez-Fernandez, Jose Luis Fernandez, Martha I Davila-Rodriguez, Stephen eJohnston, Jaime eGosalvez
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-11-01
Series:Frontiers in Genetics
Subjects:
Comet Assay
Fertility
sperm dna damage
Eutheria
Metatheria
Two-tailed Comet assay (2T-Comet)
Online Access:http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00404/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00404/full

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