Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors
Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00213/full |
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| author | Ning Jia Ning Jia Ning Jia Jiqin Liu Penghui Tan Yufeng Sun Yufeng Sun Yuemeng Lv Jiameng Liu Jiameng Liu Jing Sun Jing Sun Yatao Huang Yatao Huang Jia Lu Jia Lu Nuo Jin Nuo Jin Minmin Li Minmin Li Khandaker Md Sharif Uddin Imam Fengjiao Xin Bei Fan Bei Fan |
| author_facet | Ning Jia Ning Jia Ning Jia Jiqin Liu Penghui Tan Yufeng Sun Yufeng Sun Yuemeng Lv Jiameng Liu Jiameng Liu Jing Sun Jing Sun Yatao Huang Yatao Huang Jia Lu Jia Lu Nuo Jin Nuo Jin Minmin Li Minmin Li Khandaker Md Sharif Uddin Imam Fengjiao Xin Bei Fan Bei Fan |
| author_sort | Ning Jia |
| collection | DOAJ |
| description | Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate MYB transcription factor, CsMYB85, that is involved in the regulation of lignin biosynthesis in Citrus sinensis, which has homologs in Arabidopsis and other plants. We found that during juice sac lignification, CsMYB85 expression levels increase significantly, and therefore, suspected that this gene may control lignin biosynthesis during the lignification process. Our results indicated that CsMYB85 binds the CsMYB330 promoter, regulates its expression, and interacts with CsMYB308 in transgenic yeast and tobacco. A transient expression assay indicated that Cs4CL1 expression levels and lignin content significantly increased in fruit juice sacs overexpressing CsMYB85. At4CL1 expression levels and lignin content were also significantly increased in Arabidopsis overexpressing CsMYB85. We accordingly present convincing evidence for the participation of the CsMYB85 transcription factor in fruit juice sac lignification, and thereby provide new insights into the transcriptional regulation of this process in citrus fruits. |
| first_indexed | 2024-12-20T04:32:26Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T04:32:26Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-8a7dfce990fb47a4bc396871449932f12022-12-21T19:53:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-02-011010.3389/fpls.2019.00213434878Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription FactorsNing Jia0Ning Jia1Ning Jia2Jiqin Liu3Penghui Tan4Yufeng Sun5Yufeng Sun6Yuemeng Lv7Jiameng Liu8Jiameng Liu9Jing Sun10Jing Sun11Yatao Huang12Yatao Huang13Jia Lu14Jia Lu15Nuo Jin16Nuo Jin17Minmin Li18Minmin Li19Khandaker Md Sharif Uddin Imam20Fengjiao Xin21Bei Fan22Bei Fan23Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaQinhuangdao Customs, Hebei Qinhuangdao, Qinhuangdao, ChinaTurfgrass Research Institute, Beijing Forestry University, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Quality and Safety Risk Assessment on Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing, ChinaVarieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate MYB transcription factor, CsMYB85, that is involved in the regulation of lignin biosynthesis in Citrus sinensis, which has homologs in Arabidopsis and other plants. We found that during juice sac lignification, CsMYB85 expression levels increase significantly, and therefore, suspected that this gene may control lignin biosynthesis during the lignification process. Our results indicated that CsMYB85 binds the CsMYB330 promoter, regulates its expression, and interacts with CsMYB308 in transgenic yeast and tobacco. A transient expression assay indicated that Cs4CL1 expression levels and lignin content significantly increased in fruit juice sacs overexpressing CsMYB85. At4CL1 expression levels and lignin content were also significantly increased in Arabidopsis overexpressing CsMYB85. We accordingly present convincing evidence for the participation of the CsMYB85 transcription factor in fruit juice sac lignification, and thereby provide new insights into the transcriptional regulation of this process in citrus fruits.https://www.frontiersin.org/article/10.3389/fpls.2019.00213/fullCitrus sinensisCsMYB85juice sacslignificationpostharvest |
| spellingShingle | Ning Jia Ning Jia Ning Jia Jiqin Liu Penghui Tan Yufeng Sun Yufeng Sun Yuemeng Lv Jiameng Liu Jiameng Liu Jing Sun Jing Sun Yatao Huang Yatao Huang Jia Lu Jia Lu Nuo Jin Nuo Jin Minmin Li Minmin Li Khandaker Md Sharif Uddin Imam Fengjiao Xin Bei Fan Bei Fan Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors Frontiers in Plant Science Citrus sinensis CsMYB85 juice sacs lignification postharvest |
| title | Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors |
| title_full | Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors |
| title_fullStr | Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors |
| title_full_unstemmed | Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors |
| title_short | Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors |
| title_sort | citrus sinensis myb transcription factor csmyb85 induce fruit juice sac lignification through interaction with other csmyb transcription factors |
| topic | Citrus sinensis CsMYB85 juice sacs lignification postharvest |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00213/full |
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