Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.)

Abstract Quantitative real-time polymerase chain reaction (qRT-PCR) is a sensitive and widely used technique for quantifying gene expression levels, and its accuracy depends on the reference genes used for data normalization. To date, no reference gene has been reported in the nutritious and functio...

Full description

Bibliographic Details
Main Authors: Jing-Rong Zhang, Yuan-Yuan Feng, Ma-Jin Yang, Yu Xiao, Yu-Shan Liu, Yuan Yuan, Zhen Li, Yan Zhang, Ming Zhuo, Jun Zhang, Cai-Xia Li
Format: Article
Language:English
Published: Nature Portfolio 2022-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-16124-3