Stability of feed enzymes in physiological conditions assayed by in vitro methods
A series of in vitro incubations were carried out to investigate the stability of two enzyme preparations in conditions similar to those in the upper gastrointestinal tract of monogastric animals. The two enzyme products, one crude xylanase from Trichoderma longibrachiatum (Multifekt K) and the othe...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Scientific Agricultural Society of Finland
1993-03-01
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| Series: | Agricultural and Food Science |
| Online Access: | https://journal.fi/afs/article/view/72479 |
| _version_ | 1811276468432404480 |
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| author | Johan Inborr Anne Grönlund |
| author_facet | Johan Inborr Anne Grönlund |
| author_sort | Johan Inborr |
| collection | DOAJ |
| description | A series of in vitro incubations were carried out to investigate the stability of two enzyme preparations in conditions similar to those in the upper gastrointestinal tract of monogastric animals. The two enzyme products, one crude xylanase from Trichoderma longibrachiatum (Multifekt K) and the other a specifically manufactured feed enzyme (Avizyme SX®), were subjected to incubations at low and neutral pH with and without proteolytic enzymes (pepsin and pancreatin). Wheat gluten was employed together with the crude xylanase to investigate its potential as a stabilising agent. Due to the buffering effect of Avizyme SX®, incubations were carried out with (pH 2.5) and without (pH 3.2) addition of either citric or hydrochloric acid. Incubation of the crude xylanase at low pH followed by incubation at neutral pH resulted in negligible loss of xylanase activity whereas β-xylosidase recovery fell to 57 per cent of the initial value (P |
| first_indexed | 2024-04-12T23:57:59Z |
| format | Article |
| id | doaj.art-8aae3b43d6bb4ccea59c1f8b65009f7d |
| institution | Directory Open Access Journal |
| issn | 1459-6067 1795-1895 |
| language | English |
| last_indexed | 2024-04-12T23:57:59Z |
| publishDate | 1993-03-01 |
| publisher | Scientific Agricultural Society of Finland |
| record_format | Article |
| series | Agricultural and Food Science |
| spelling | doaj.art-8aae3b43d6bb4ccea59c1f8b65009f7d2022-12-22T03:11:26ZengScientific Agricultural Society of FinlandAgricultural and Food Science1459-60671795-18951993-03-0122Stability of feed enzymes in physiological conditions assayed by in vitro methodsJohan Inborr0Anne GrönlundFinnfeeds International Ltd., Market House, High Street, Marlborough, Wiltshire SNB IAA, United KingdomA series of in vitro incubations were carried out to investigate the stability of two enzyme preparations in conditions similar to those in the upper gastrointestinal tract of monogastric animals. The two enzyme products, one crude xylanase from Trichoderma longibrachiatum (Multifekt K) and the other a specifically manufactured feed enzyme (Avizyme SX®), were subjected to incubations at low and neutral pH with and without proteolytic enzymes (pepsin and pancreatin). Wheat gluten was employed together with the crude xylanase to investigate its potential as a stabilising agent. Due to the buffering effect of Avizyme SX®, incubations were carried out with (pH 2.5) and without (pH 3.2) addition of either citric or hydrochloric acid. Incubation of the crude xylanase at low pH followed by incubation at neutral pH resulted in negligible loss of xylanase activity whereas β-xylosidase recovery fell to 57 per cent of the initial value (Phttps://journal.fi/afs/article/view/72479 |
| spellingShingle | Johan Inborr Anne Grönlund Stability of feed enzymes in physiological conditions assayed by in vitro methods Agricultural and Food Science |
| title | Stability of feed enzymes in physiological conditions assayed by in vitro methods |
| title_full | Stability of feed enzymes in physiological conditions assayed by in vitro methods |
| title_fullStr | Stability of feed enzymes in physiological conditions assayed by in vitro methods |
| title_full_unstemmed | Stability of feed enzymes in physiological conditions assayed by in vitro methods |
| title_short | Stability of feed enzymes in physiological conditions assayed by in vitro methods |
| title_sort | stability of feed enzymes in physiological conditions assayed by in vitro methods |
| url | https://journal.fi/afs/article/view/72479 |
| work_keys_str_mv | AT johaninborr stabilityoffeedenzymesinphysiologicalconditionsassayedbyinvitromethods AT annegronlund stabilityoffeedenzymesinphysiologicalconditionsassayedbyinvitromethods |