Development of a markerless gene deletion strategy using rpsL as a counterselectable marker and characterization of the function of RA0C_1534 in Riemerella anatipestifer ATCC11845 using this strategy.

Development of a markerless gene deletion strategy using rpsL as a counterselectable marker and characterization of the function of RA0C_1534 in Riemerella anatipestifer ATCC11845 using this strategy.

Riemerella anatipestifer is a gram-negative bacterium that mainly infects ducks, turkeys and other birds. In a previous study, we established a markerless mutation system based on the pheS mutant as a counterselectable marker. However, the toxic effect of p-Cl-Phe on the R. anatipestifer strain expr...

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Bibliographic Details
Main Authors: MaFeng Liu, Xiu Tian, MengYi Wang, DeKang Zhu, MingShu Wang, RenYong Jia, Shun Chen, XinXin Zhao, Qiao Yang, Ying Wu, ShaQiu Zhang, Juan Huang, Bin Tian, XiaoYue Chen, YunYa Liu, Ling Zhang, YanLing Yu, Francis Biville, LeiChang Pan, Mujeeb Ur Rehman, AnChun Cheng
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0218241

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https://doi.org/10.1371/journal.pone.0218241

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