IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection.
Type 2 immune responses are essential in protection against intestinal helminth infections. In this study we show that IL-22, a cytokine important in defence against bacterial infections in the intestinal tract, is also a critical mediator of anti-helminth immunity. After infection with Nippostrongy...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC3795034?pdf=render |
| _version_ | 1818253665624915968 |
|---|---|
| author | Jan-Eric Turner Brigitta Stockinger Helena Helmby |
| author_facet | Jan-Eric Turner Brigitta Stockinger Helena Helmby |
| author_sort | Jan-Eric Turner |
| collection | DOAJ |
| description | Type 2 immune responses are essential in protection against intestinal helminth infections. In this study we show that IL-22, a cytokine important in defence against bacterial infections in the intestinal tract, is also a critical mediator of anti-helminth immunity. After infection with Nippostrongylus brasiliensis, a rodent hookworm, IL-22-deficient mice showed impaired worm expulsion despite normal levels of type 2 cytokine production. The impaired worm expulsion correlated with reduced goblet cell hyperplasia and reduced expression of goblet cell markers. We further confirmed our findings in a second nematode model, the murine whipworm Trichuris muris. T.muris infected IL-22-deficient mice had a similar phenotype to that seen in N.brasiliensis infection, with impaired worm expulsion and reduced goblet cell hyperplasia. Ex vivo and in vitro analysis demonstrated that IL-22 is able to directly induce the expression of several goblet cell markers, including mucins. Taken together, our findings reveal that IL-22 plays an important role in goblet cell activation, and thus, a key role in anti-helminth immunity. |
| first_indexed | 2024-12-12T16:43:41Z |
| format | Article |
| id | doaj.art-8ac3bf5a58524c30baadb22743d85be6 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-12T16:43:41Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-8ac3bf5a58524c30baadb22743d85be62022-12-22T00:18:31ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742013-01-01910e100369810.1371/journal.ppat.1003698IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection.Jan-Eric TurnerBrigitta StockingerHelena HelmbyType 2 immune responses are essential in protection against intestinal helminth infections. In this study we show that IL-22, a cytokine important in defence against bacterial infections in the intestinal tract, is also a critical mediator of anti-helminth immunity. After infection with Nippostrongylus brasiliensis, a rodent hookworm, IL-22-deficient mice showed impaired worm expulsion despite normal levels of type 2 cytokine production. The impaired worm expulsion correlated with reduced goblet cell hyperplasia and reduced expression of goblet cell markers. We further confirmed our findings in a second nematode model, the murine whipworm Trichuris muris. T.muris infected IL-22-deficient mice had a similar phenotype to that seen in N.brasiliensis infection, with impaired worm expulsion and reduced goblet cell hyperplasia. Ex vivo and in vitro analysis demonstrated that IL-22 is able to directly induce the expression of several goblet cell markers, including mucins. Taken together, our findings reveal that IL-22 plays an important role in goblet cell activation, and thus, a key role in anti-helminth immunity.http://europepmc.org/articles/PMC3795034?pdf=render |
| spellingShingle | Jan-Eric Turner Brigitta Stockinger Helena Helmby IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection. PLoS Pathogens |
| title | IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection. |
| title_full | IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection. |
| title_fullStr | IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection. |
| title_full_unstemmed | IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection. |
| title_short | IL-22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection. |
| title_sort | il 22 mediates goblet cell hyperplasia and worm expulsion in intestinal helminth infection |
| url | http://europepmc.org/articles/PMC3795034?pdf=render |
| work_keys_str_mv | AT janericturner il22mediatesgobletcellhyperplasiaandwormexpulsioninintestinalhelminthinfection AT brigittastockinger il22mediatesgobletcellhyperplasiaandwormexpulsioninintestinalhelminthinfection AT helenahelmby il22mediatesgobletcellhyperplasiaandwormexpulsioninintestinalhelminthinfection |