Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model
Nanohydrogels show great potential as efficient drug carriers due to their biocompatibility, low toxicity, and high water absorbability. In this paper, we prepared two O-carboxymethylated chitosan (OCMC)-based polymers functionalized with β-cyclodextrin (β-CD) and amino acid. The structures of the p...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-05-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1149191/full |
| _version_ | 1797829346849718272 |
|---|---|
| author | Li Cui Li Cui Xiaolan Liu Rongjun Yan Qixu Chen Lizhen Wang Shah Nawaz Dawei Qin Daijie Wang Daijie Wang |
| author_facet | Li Cui Li Cui Xiaolan Liu Rongjun Yan Qixu Chen Lizhen Wang Shah Nawaz Dawei Qin Daijie Wang Daijie Wang |
| author_sort | Li Cui |
| collection | DOAJ |
| description | Nanohydrogels show great potential as efficient drug carriers due to their biocompatibility, low toxicity, and high water absorbability. In this paper, we prepared two O-carboxymethylated chitosan (OCMC)-based polymers functionalized with β-cyclodextrin (β-CD) and amino acid. The structures of the polymers were characterized by Fourier Transform Infrared (FTIR) Spectroscopy. Morphological study was carried out on a Transmission Electron Microscope (TEM), and the results indicated that the two polymers had irregular spheroidal structure with some pores distributed on their surface. The average particle diameter was below 500 nm, and the zeta potential was above +30 mV. The two polymers were further used for preparing nanohydrogels loaded with anticancer drugs lapatinib and ginsenoside Rg1, and the resulting nanohydrogels showed high drug loading efficiency and pH-sensitive (pH = 4.5) drug release behavior. In vitro cytotoxicity investigation revealed that the nanohydrogels exhibited high cytotoxicity against lung cancer (A549) cells. In vivo anticancer investigation was performed in a transgenic Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasV12) zebrafish model. The results showed that the synthesized nanohydrogels significantly inhibited the expression of EGFP-krasv12 oncogene in zebrafish liver, and the L-arginine modified OCMC-g-Suc-β-CD nanohydrogels loading lapatinib and ginsenoside Rg1 showed the best results. |
| first_indexed | 2024-04-09T13:19:00Z |
| format | Article |
| id | doaj.art-8ac78c3cebbc496b8c31eaeaa5d9af8f |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-09T13:19:00Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-8ac78c3cebbc496b8c31eaeaa5d9af8f2023-05-11T11:18:26ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-05-011410.3389/fphar.2023.11491911149191Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish modelLi Cui0Li Cui1Xiaolan Liu2Rongjun Yan3Qixu Chen4Lizhen Wang5Shah Nawaz6Dawei Qin7Daijie Wang8Daijie Wang9School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaShandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaJinan Authority Hospital, Jinan, ChinaJinan International Travel Healthcare Center, Jinan, ChinaBiological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaDepartment of Chemistry, Karakoram International University, Gilgit, PakistanSchool of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaShandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaBiological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, ChinaNanohydrogels show great potential as efficient drug carriers due to their biocompatibility, low toxicity, and high water absorbability. In this paper, we prepared two O-carboxymethylated chitosan (OCMC)-based polymers functionalized with β-cyclodextrin (β-CD) and amino acid. The structures of the polymers were characterized by Fourier Transform Infrared (FTIR) Spectroscopy. Morphological study was carried out on a Transmission Electron Microscope (TEM), and the results indicated that the two polymers had irregular spheroidal structure with some pores distributed on their surface. The average particle diameter was below 500 nm, and the zeta potential was above +30 mV. The two polymers were further used for preparing nanohydrogels loaded with anticancer drugs lapatinib and ginsenoside Rg1, and the resulting nanohydrogels showed high drug loading efficiency and pH-sensitive (pH = 4.5) drug release behavior. In vitro cytotoxicity investigation revealed that the nanohydrogels exhibited high cytotoxicity against lung cancer (A549) cells. In vivo anticancer investigation was performed in a transgenic Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasV12) zebrafish model. The results showed that the synthesized nanohydrogels significantly inhibited the expression of EGFP-krasv12 oncogene in zebrafish liver, and the L-arginine modified OCMC-g-Suc-β-CD nanohydrogels loading lapatinib and ginsenoside Rg1 showed the best results.https://www.frontiersin.org/articles/10.3389/fphar.2023.1149191/fullOCMC-g-β-CD nanohydrogelslapatinibginsenoside Rg1anticancer activityzebrafish |
| spellingShingle | Li Cui Li Cui Xiaolan Liu Rongjun Yan Qixu Chen Lizhen Wang Shah Nawaz Dawei Qin Daijie Wang Daijie Wang Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model Frontiers in Pharmacology OCMC-g-β-CD nanohydrogels lapatinib ginsenoside Rg1 anticancer activity zebrafish |
| title | Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model |
| title_full | Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model |
| title_fullStr | Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model |
| title_full_unstemmed | Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model |
| title_short | Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model |
| title_sort | amino acid modified ocmc g suc β cd nanohydrogels carrying lapatinib and ginsenoside rg1 exhibit high anticancer activity in a zebrafish model |
| topic | OCMC-g-β-CD nanohydrogels lapatinib ginsenoside Rg1 anticancer activity zebrafish |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1149191/full |
| work_keys_str_mv | AT licui aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT licui aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT xiaolanliu aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT rongjunyan aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT qixuchen aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT lizhenwang aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT shahnawaz aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT daweiqin aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT daijiewang aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel AT daijiewang aminoacidmodifiedocmcgsucbcdnanohydrogelscarryinglapatinibandginsenosiderg1exhibithighanticanceractivityinazebrafishmodel |