A protocol to study bacteriophage adaptation to new hosts

A protocol to study bacteriophage adaptation to new hosts

Summary: A general protocol for the experimental assessment of bacteriophage adaptation to new hosts is described. We use as a model system the lytic phage T7 and an engineered E. coli strain modified to hamper the recruitment of a known proviral factor. Our protocol includes steps of phage amplific...

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Bibliographic Details
Main Authors: Raquel Luzon-Hidalgo, Valeria A. Risso, Asuncion Delgado, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Biophysics
Microbiology
Model organisms
Molecular biology
Biotechnology and bioengineering
Evolutionary biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004901

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http://www.sciencedirect.com/science/article/pii/S2666166721004901

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