| Summary: | Wheat is a worldwide staple food crop, and take-all caused by <i>Gaeumannomyces graminis</i> var. <i>tritici</i> can lead to a tremendous decrease in wheat yield and quality. In this study, strain YB-10 was isolated from wheat rhizospheric soil and identified as <i>Pseudomonas chlororaphis</i> by morphology and 16S rRNA gene sequencing. <i>Pseudomonas chlororaphis</i> YB-10 had extracellular protease and cellulase activities and strongly inhibited the mycelium growth of <i>Gaeumannomyces graminis</i> var. <i>tritici</i> in dual cultures. Up to 87% efficacy of <i>Pseudomonas chlororaphis</i> YB-10 in controlling the take-all of seedlings was observed in pot experiments when wheat seed was coated with the bacterium. <i>Pseudomonas chlororaphis</i> YB-10 was also positive for indole acetic acid (IAA) and siderophore production, and coating wheat seed with the bacterium significantly promoted the growth of seedlings at 10<sup>7</sup> and 10<sup>8</sup> CFU/mL. Furthermore, treatment with <i>Pseudomonas chlororaphis</i> YB-10 increased activities of the wheat defense-related enzymes POD, SOD, CAT, PAL and PPO in seedlings, indicating induced resistance against pathogens. Overall, <i>Pseudomonas chlororaphis</i> YB-10 is a promising new seed-coating agent to both promote wheat growth and suppress take-all.
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