Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep

Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep

Abstract Background The simplicity of the CRISPR/Cas9 system has enabled its widespread applications in generating animal models, functional genomic screening and in treating genetic and infectious diseases. However, unintended mutations produced by off-target CRISPR/Cas9 nuclease activity may lead...

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Bibliographic Details
Main Authors: Xiaolong Wang, Jing Liu, Yiyuan Niu, Yan Li, Shiwei Zhou, Chao Li, Baohua Ma, Qifang Kou, Bjoern Petersen, Tad Sonstegard, Xingxu Huang, Yu Jiang, Yulin Chen
Format: Article
Language:English
Published: BMC 2018-05-01
Series:BMC Genomics
Subjects:
Genome editing
CRISPR/Cas9
Whole genome sequencing
Off-target; mutation rate
Sheep
Online Access:http://link.springer.com/article/10.1186/s12864-018-4712-z

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http://link.springer.com/article/10.1186/s12864-018-4712-z

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