A New Phospholipase D from <i>Moritella</i> sp. JT01: Biochemical Characterization, Crystallization and Application in the Synthesis of Phosphatidic Acid

A New Phospholipase D from <i>Moritella</i> sp. JT01: Biochemical Characterization, Crystallization and Application in the Synthesis of Phosphatidic Acid

A new phospholipase D from marine <i>Moritella</i> sp. JT01 (MsPLD) was recombinantly expressed and biochemically characterized. The optimal reaction temperature and pH of MsPLD were determined to be 35 °C and 8.0. MsPLD was stable at a temperature lower than 35 °C, and the t<sub>1...

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Bibliographic Details
Main Authors: Fanghua Wang, Xuejing Mao, Fuli Deng, Ruiguo Cui, Lilang Li, Siyu Liu, Bo Yang, Dongming Lan, Yonghua Wang
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:International Journal of Molecular Sciences
Subjects:
phospholipase D
phosphatidic acid
crystal structure
<i>Moritella</i> sp. JT01
monolayer technology
maximum insertion pressure
Online Access:https://www.mdpi.com/1422-0067/23/19/11633

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https://www.mdpi.com/1422-0067/23/19/11633

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