High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion

High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion

Precise and efficient insertion of large DNA fragments into somatic cells using gene editing technologies to label or modify endogenous proteins remains challenging. Non-specific insertions/deletions (INDELs) resulting from the non-homologous end joining pathway make the process error-prone. Further...

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Bibliographic Details
Main Authors: Haining Zhong, Cesar C Ceballos, Crystian I Massengill, Michael A Muniak, Lei Ma, Maozhen Qin, Stefanie Kaech Petrie, Tianyi Mao
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-06-01
Series:eLife
Subjects:
CRISPR/Cas9
endogenous fluorescent protein labeling
precise protein sequence insertion
cytoskeleton labeling
synaptic protein labeling
in vivo imaging
Online Access:https://elifesciences.org/articles/64911

Internet

https://elifesciences.org/articles/64911

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