Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis
Abstract Background Distraction osteogenesis (DO) is an efficacious but lengthy procedure to reconstruct segmental bone defects under the principle of tension-stress, during which the periosteum-mediated mechanical stimulation plays a pivotal role. Inspired by the dynamic process of DO and the mecha...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2022-12-01
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| Series: | Biomaterials Research |
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| Online Access: | https://doi.org/10.1186/s40824-022-00330-1 |
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| author | Tengfei Lou Kai Chen Qiyu Luo Changsheng Liu Yuan Yuan Cunyi Fan |
| author_facet | Tengfei Lou Kai Chen Qiyu Luo Changsheng Liu Yuan Yuan Cunyi Fan |
| author_sort | Tengfei Lou |
| collection | DOAJ |
| description | Abstract Background Distraction osteogenesis (DO) is an efficacious but lengthy procedure to reconstruct segmental bone defects under the principle of tension-stress, during which the periosteum-mediated mechanical stimulation plays a pivotal role. Inspired by the dynamic process of DO and the mechanical stimulation of periosteum, a new design of bionic periosteum was developed to simulate the mechanical transduction of natural periosteum for the application in DO procedure. Methods In this study, an injectable organic-inorganic hybrid hydrogel was developed based on a novel combination of the PEGylated poly (glycerol sebacate) (PEGS) polymer network and in situ formed CaP nanoparticles (ICPNs). Rat bone marrow mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured and tested in vitro to evaluate biocompatibility, cell adhesion, proliferation, and pro-osteogenic and pro-angiogenic activity. In vivo experiments were conducted in the rat tibial model of distraction osteogenesis. Results The developed nanocomposite hydrogels exhibited excellent injectability, robust bone adhesion, superior stretchability, and enhanced osteogenic activity. The results of in vitro and in vivo studies showed that PEGS/ICPN hydrogels could promote new bone formation and mineralization during the dynamic distraction process through the synergistic effects of angiogenesis and osteogenesis. Conclusions This periosteum-inspired nanocomposite hydrogel represents a mechanobiology approach for effectively restoring large bone defects through the dynamic DO process. |
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| id | doaj.art-8b3cafb1c1cd494a94a5308a11e5597c |
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| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T18:33:13Z |
| publishDate | 2022-12-01 |
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| series | Biomaterials Research |
| spelling | doaj.art-8b3cafb1c1cd494a94a5308a11e5597c2024-03-02T05:41:00ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242022-12-0126112210.1186/s40824-022-00330-1Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesisTengfei Lou0Kai Chen1Qiyu Luo2Changsheng Liu3Yuan Yuan4Cunyi Fan5Orthopaedic Department, Shanghai Sixth People’s HospitalKey Laboratory for Ultrafine Materials of Ministry of Education, and School of Materials Science and Engineering, East China University of Science and TechnologyOrthopaedic Department, Shanghai Sixth People’s HospitalKey Laboratory for Ultrafine Materials of Ministry of Education, and School of Materials Science and Engineering, East China University of Science and TechnologyKey Laboratory for Ultrafine Materials of Ministry of Education, and School of Materials Science and Engineering, East China University of Science and TechnologyOrthopaedic Department, Shanghai Sixth People’s HospitalAbstract Background Distraction osteogenesis (DO) is an efficacious but lengthy procedure to reconstruct segmental bone defects under the principle of tension-stress, during which the periosteum-mediated mechanical stimulation plays a pivotal role. Inspired by the dynamic process of DO and the mechanical stimulation of periosteum, a new design of bionic periosteum was developed to simulate the mechanical transduction of natural periosteum for the application in DO procedure. Methods In this study, an injectable organic-inorganic hybrid hydrogel was developed based on a novel combination of the PEGylated poly (glycerol sebacate) (PEGS) polymer network and in situ formed CaP nanoparticles (ICPNs). Rat bone marrow mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured and tested in vitro to evaluate biocompatibility, cell adhesion, proliferation, and pro-osteogenic and pro-angiogenic activity. In vivo experiments were conducted in the rat tibial model of distraction osteogenesis. Results The developed nanocomposite hydrogels exhibited excellent injectability, robust bone adhesion, superior stretchability, and enhanced osteogenic activity. The results of in vitro and in vivo studies showed that PEGS/ICPN hydrogels could promote new bone formation and mineralization during the dynamic distraction process through the synergistic effects of angiogenesis and osteogenesis. Conclusions This periosteum-inspired nanocomposite hydrogel represents a mechanobiology approach for effectively restoring large bone defects through the dynamic DO process.https://doi.org/10.1186/s40824-022-00330-1In situ precipitated CaP nanoparticlesNanocomposite hydrogelsBone adhesionStretchabilityDynamic distraction osteogenesisMechanical stimulation |
| spellingShingle | Tengfei Lou Kai Chen Qiyu Luo Changsheng Liu Yuan Yuan Cunyi Fan Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis Biomaterials Research In situ precipitated CaP nanoparticles Nanocomposite hydrogels Bone adhesion Stretchability Dynamic distraction osteogenesis Mechanical stimulation |
| title | Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis |
| title_full | Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis |
| title_fullStr | Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis |
| title_full_unstemmed | Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis |
| title_short | Periosteum-inspired in situ CaP generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis |
| title_sort | periosteum inspired in situ cap generated nanocomposite hydrogels with strong bone adhesion and superior stretchability for accelerated distraction osteogenesis |
| topic | In situ precipitated CaP nanoparticles Nanocomposite hydrogels Bone adhesion Stretchability Dynamic distraction osteogenesis Mechanical stimulation |
| url | https://doi.org/10.1186/s40824-022-00330-1 |
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