Analysis of Wild Type LbCpf1 Protein, and PAM Recognition Variants, in a Cellular Context

Analysis of Wild Type LbCpf1 Protein, and PAM Recognition Variants, in a Cellular Context

Nucleases used in genome engineering induce hydrolysis of DNA phosphate backbone in a sequence-specific manner. So far CRISPR-Cas, the RNA-guided nucleases, is the most advanced genome engineering system. The CRISPR nucleases allows recognition of a particular genomic sequence with two distinct mole...

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Bibliographic Details
Main Authors: Ujin Shin, Vincent Brondani
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Genetics
Subjects:
Cpf1
genome engineering
PAM
specificity
selectivity
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2020.571591/full

Internet

https://www.frontiersin.org/articles/10.3389/fgene.2020.571591/full

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