Heterologous Expression of Extracellular Proteinase pAsPs of <i>Aspergillus pseudotamarii</i> in <i>Komagataella phaffii</i>

Heterologous Expression of Extracellular Proteinase pAsPs of <i>Aspergillus pseudotamarii</i> in <i>Komagataella phaffii</i>

Neutral protease pAsPs gene was obtained by sequence optimization of NpI protease from <i>Aspergillus pseudotamarii.</i> pAsPs was for the first time integrated in the genome of yeast strain <i>Komagataella phaffii</i> T07, and then produced in a 5 L bioreactor with an enzyme...

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Main Authors: Andrey Valentinovich Zadorozhny, Mikhail Evgenyevich Voskoboev, Denis Vladimirovich Bochkov, Alexei Sergeyevich Rozanov, Elizaveta Dmitrievna Shedko, Irina Anatolyevna Mescheryakova, Alexander Gennadyevich Blinov, Anton Vladimirovich Korzhuk, Valeria Nikolayevna Shlyakhtun, Natalia Vladimirovna Bogacheva, Egor Vladimirovich Antonov, Svetlana Valerevna Bannikova, Tatiana Nikolayevna Goryachkovskaya, Sergey Evgenyevich Peltek
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
proteinase
<i>Komagataella phaffii</i>
multicopy
heterologous expression
<i>Aspergillus pseudotamarii</i>
industrial application
Online Access:https://www.mdpi.com/1422-0067/23/23/15035

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https://www.mdpi.com/1422-0067/23/23/15035

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