Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells

Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells

Summary: Immune synapse (IS) formation determines T cell antitumor activity. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging. We describe steps for CAR T cel...

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Bibliographic Details
Main Authors: Jorge Ibanez, Nikhil Hebbar, Unmesha Thanekar, Zhongzhen Yi, Haley Houke, Meghan Ward, Chris Nevitt, Liqing Tian, Stephen C. Mack, Heather Sheppard, Jason Chiang, M. Paulina Velasquez, Giedre Krenciute
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
cancer
immunology
microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005872

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724005872

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