Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells

Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells

Summary: Immune synapse (IS) formation determines T cell antitumor activity. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging. We describe steps for CAR T cel...

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Chi tiết về thư mục
Những tác giả chính:	Jorge Ibanez, Nikhil Hebbar, Unmesha Thanekar, Zhongzhen Yi, Haley Houke, Meghan Ward, Chris Nevitt, Liqing Tian, Stephen C. Mack, Heather Sheppard, Jason Chiang, M. Paulina Velasquez, Giedre Krenciute
Định dạng:	Bài viết
Ngôn ngữ:	English
Được phát hành:	Elsevier 2024-12-01
Loạt:	STAR Protocols
Những chủ đề:	cancer immunology microscopy
Truy cập trực tuyến:	http://www.sciencedirect.com/science/article/pii/S2666166724005872

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