Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells
Summary: Immune synapse (IS) formation determines T cell antitumor activity. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging. We describe steps for CAR T cel...
| मुख्य लेखकों: | , , , , , , , , , , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
Elsevier
2024-12-01
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| श्रृंखला: | STAR Protocols |
| विषय: | |
| ऑनलाइन पहुंच: | http://www.sciencedirect.com/science/article/pii/S2666166724005872 |