Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers
<p>Abstract</p> <p>Background</p> <p>The strenuous procurement of cultured human hepatocytes and their short lives have constrained the cell culture model of cytochrome P450 (CYP450) induction, xenobiotic biotransformation, and hepatotoxicity. The development of continu...
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | BMC Biotechnology |
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| Online Access: | http://www.biomedcentral.com/1472-6750/11/89 |
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| author | Limsrichamrern Somchai Akarasereenont Pravit Borwornpinyo Suparerk Nuntakarn Lalana Duangsa-ard Sunisa Kasetsinsombat Kanda Wongkajornsilp Adisak Sa-ngiamsuntorn Khanit Hongeng Suradej |
| author_facet | Limsrichamrern Somchai Akarasereenont Pravit Borwornpinyo Suparerk Nuntakarn Lalana Duangsa-ard Sunisa Kasetsinsombat Kanda Wongkajornsilp Adisak Sa-ngiamsuntorn Khanit Hongeng Suradej |
| author_sort | Limsrichamrern Somchai |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The strenuous procurement of cultured human hepatocytes and their short lives have constrained the cell culture model of cytochrome P450 (CYP450) induction, xenobiotic biotransformation, and hepatotoxicity. The development of continuous non-tumorous cell line steadily containing hepatocyte phenotypes would substitute the primary hepatocytes for these studies.</p> <p>Results</p> <p>The hepatocyte-like cells have been developed from hTERT plus Bmi-1-immortalized human mesenchymal stem cells to substitute the primary hepatocytes. The hepatocyte-like cells had polygonal morphology and steadily produced albumin, glycogen, urea and UGT1A1 beyond 6 months while maintaining proliferative capacity. Although these hepatocyte-like cells had low basal expression of CYP450 isotypes, their expressions could be extensively up regulated to 80 folds upon the exposure to enzyme inducers. Their inducibility outperformed the classical HepG2 cells.</p> <p>Conclusion</p> <p>The hepatocyte-like cells contained the markers of hepatocytes including CYP450 isotypes. The high inducibility of CYP450 transcripts could serve as a sensitive model for profiling xenobiotic-induced expression of CYP450.</p> |
| first_indexed | 2024-12-21T07:44:02Z |
| format | Article |
| id | doaj.art-8b9c6c070b7344f1864b2bf8a375f81e |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-21T07:44:02Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-8b9c6c070b7344f1864b2bf8a375f81e2022-12-21T19:11:14ZengBMCBMC Biotechnology1472-67502011-09-011118910.1186/1472-6750-11-89Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducersLimsrichamrern SomchaiAkarasereenont PravitBorwornpinyo SuparerkNuntakarn LalanaDuangsa-ard SunisaKasetsinsombat KandaWongkajornsilp AdisakSa-ngiamsuntorn KhanitHongeng Suradej<p>Abstract</p> <p>Background</p> <p>The strenuous procurement of cultured human hepatocytes and their short lives have constrained the cell culture model of cytochrome P450 (CYP450) induction, xenobiotic biotransformation, and hepatotoxicity. The development of continuous non-tumorous cell line steadily containing hepatocyte phenotypes would substitute the primary hepatocytes for these studies.</p> <p>Results</p> <p>The hepatocyte-like cells have been developed from hTERT plus Bmi-1-immortalized human mesenchymal stem cells to substitute the primary hepatocytes. The hepatocyte-like cells had polygonal morphology and steadily produced albumin, glycogen, urea and UGT1A1 beyond 6 months while maintaining proliferative capacity. Although these hepatocyte-like cells had low basal expression of CYP450 isotypes, their expressions could be extensively up regulated to 80 folds upon the exposure to enzyme inducers. Their inducibility outperformed the classical HepG2 cells.</p> <p>Conclusion</p> <p>The hepatocyte-like cells contained the markers of hepatocytes including CYP450 isotypes. The high inducibility of CYP450 transcripts could serve as a sensitive model for profiling xenobiotic-induced expression of CYP450.</p>http://www.biomedcentral.com/1472-6750/11/89hepatocyte-like cellimmortalizationCYP450MSC |
| spellingShingle | Limsrichamrern Somchai Akarasereenont Pravit Borwornpinyo Suparerk Nuntakarn Lalana Duangsa-ard Sunisa Kasetsinsombat Kanda Wongkajornsilp Adisak Sa-ngiamsuntorn Khanit Hongeng Suradej Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers BMC Biotechnology hepatocyte-like cell immortalization CYP450 MSC |
| title | Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers |
| title_full | Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers |
| title_fullStr | Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers |
| title_full_unstemmed | Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers |
| title_short | Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers |
| title_sort | upregulation of cyp 450s expression of immortalized hepatocyte like cells derived from mesenchymal stem cells by enzyme inducers |
| topic | hepatocyte-like cell immortalization CYP450 MSC |
| url | http://www.biomedcentral.com/1472-6750/11/89 |
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