Metabolic engineering using iterative self-cloning to improve lipid productivity in Coccomyxa
Abstract We previously developed a self-cloning system that introduces cDNA of the uridine monophosphate synthase gene (cUMPS) of Coccomyxa sp. strain Obi as a selectable marker into uracil-auxotrophic mutants (Ura−) of the same alga. Here, we developed a Cre/loxP-based system for the removal of cUM...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-30254-7 |