Voltage Imaging in Drosophila Using a Hybrid Chemical-Genetic Rhodamine Voltage Reporter

Voltage Imaging in Drosophila Using a Hybrid Chemical-Genetic Rhodamine Voltage Reporter

We combine a chemically-synthesized, voltage-sensitive fluorophore with a genetically encoded, self-labeling enzyme to enable voltage imaging in Drosophila melanogaster. Previously, we showed that a rhodamine voltage reporter (RhoVR) combined with the HaloTag self-labeling enzyme could be used to mo...

Full description

Bibliographic Details
Main Authors: Molly J. Kirk, Brittany R. Benlian, Yifu Han, Arya Gold, Ashvin Ravi, Parker E. Deal, Rosana S. Molina, Mikhail Drobizhev, Dion Dickman, Kristin Scott, Evan W. Miller
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Neuroscience
Subjects:
imaging
fluorescence
voltage
Drosophila
neuromuscular junction (NMJ)
Online Access:https://www.frontiersin.org/articles/10.3389/fnins.2021.754027/full

Internet

https://www.frontiersin.org/articles/10.3389/fnins.2021.754027/full

Similar Items