Deuterated nucleotides as chemical probes of RNA structure: a detailed protocol for the enzymatic synthesis of a complete set of nucleotides specifically deuterated at ribose carbons
Abstract We describe here a detailed protocol for the synthesis of ribonucleotides specifically deuterated at each ribose carbon atom. We synthesized 20 specifically deuterated ribonucleotides: ATP, CTP, GTP, and UTP, each of which contained one of five deuterated riboses (either 1′-D, 2″-D, 3′-D,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
ScienceOpen
2015-04-01
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| Series: | ScienceOpen Research |
| Online Access: | https://www.scienceopen.com/document?vid=eb44f1b0-c408-4336-a2c0-aed203250898 |